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Journal of Virology, June 2009, p. 6306-6311, Vol. 83, No. 12
0022-538X/09/$08.00+0     doi:10.1128/JVI.00213-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Splicing of the Large Intron Present in the Nonstructural Gene of Minute Virus of Mice Is Governed by TIA-1/TIAR Binding Downstream of the Nonconsensus Donor{triangledown}

Eun-Young Choi{dagger} and David Pintel*

Program in Genetics and Department of Molecular Microbiology and Immunology, University of Missouri—Columbia School of Medicine, Life Sciences Center, Columbia, Missouri 65211

Received 29 January 2009/ Accepted 24 March 2009

The essential proteins NS1 and NS2 of minute virus of mice are encoded by mRNAs R1 and R2, respectively. R2 is derived from R1 by excision of a large intron and thus splicing governs the relative ratios of NS1 and NS2. Excision of the large intron utilizes a nonconsensus 5' donor site. We identified a U-rich and A-rich intronic sequence immediately downstream of the nonconsensus 5' donor site that functions as an intronic splicing enhancer (ISE) required for efficient large-intron excision. The ISE binds the cellular RNA-processing proteins TIA-1 and TIAR, which enhance usage of the nonconsensus donor.

* Corresponding author. Mailing address: 471f Life Sciences Center, University of Missouri—Columbia, 1201 Rollins Rd., Columbia, MO 65211. Phone: (573) 882-3920. Fax: (573) 884-9676. E-mail: pinteld{at}missouri.edu

{triangledown} Published ahead of print on 1 April 2009.

{dagger} Present address: Gene Therapy Center, University of North Carolina—Chapel Hill, Chapel Hill, NC 27599-7352.

Journal of Virology, June 2009, p. 6306-6311, Vol. 83, No. 12
0022-538X/09/$08.00+0     doi:10.1128/JVI.00213-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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