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Journal of Virology, June 2009, p. 5947-5950, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.00450-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Influenza A Virus Lacking M2 Protein as a Live Attenuated Vaccine{triangledown}

Shinji Watanabe,1 Tokiko Watanabe,1 and Yoshihiro Kawaoka1,2,3*

Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, Madison, Wisconsin 53706,1 International Research Center for Infectious Diseases,2 Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan3

Received 4 March 2009/ Accepted 16 March 2009

Mutant influenza virus that lacks the transmembrane and cytoplasmic tail domains of M2 (M2 knockout [M2KO]) is attenuated in both cell culture and mice. Here, we examined the potency of M2KO influenza virus as a live attenuated influenza vaccine. M2KO virus grew as efficiently as the wild-type virus in cells stably expressing the wild-type M2, indicating the feasibility of efficient vaccine production. Mice intranasally vaccinated with M2KO virus developed protective immune responses and survived a lethal challenge with the wild-type virus, suggesting that the M2KO virus has potential as a live attenuated vaccine.

* Corresponding author. Mailing address: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, 2015 Linden Drive, Madison, WI 53706. Phone: (608) 265-4925. Fax: (608) 265-5622. E-mail: kawaokay{at}svm.vetmed.wisc.edu

{triangledown} Published ahead of print on 25 March 2009.

Journal of Virology, June 2009, p. 5947-5950, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.00450-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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