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Journal of Virology, June 2009, p. 5749-5759, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.02281-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

A Novel Cardiotropic Murine Adenovirus Representing a Distinct Species of Mastadenoviruses{triangledown}

Boris Klempa,1,2 Detlev H. Krüger,1 Brita Auste,1 Michal Stanko,3 Adalbert Krawczyk,4 Katrin F. Nickel,4 Klaus Überla,4* and Alexander Stang4

Institute of Virology, Helmut-Ruska-Haus, Charité University Hospital, D-10098 Berlin, Germany,1 Institute of Virology, Dubravska cesta 9, Slovak Academy of Sciences, 84505 Bratislava, Slovakia,2 Institute of Zoology, Slovak Academy of Sciences, Dubravska cesta 9, 845 06 Bratislava, Slovakia,3 Department of Molecular and Medical Virology, Ruhr-University Bochum, D-44780 Bochum, Germany4

Received 31 October 2008/ Accepted 6 March 2009

During cell culture isolation experiments to recover Dobrava hantavirus from a suspension of liver from a striped field mouse (Apodemus agrarius), an unknown virus was coisolated. Atypically for hantaviruses, it had extensive cytopathic effects. Using a random PCR approach, it was identified as a novel murine adenovirus, MAdV-3 (for MAdV type 3). A plaque-purified virus clone was prepared and further characterized. The complete genome sequence of MAdV-3 was determined to be 30,570 bp in length. Sequence comparisons to other adenovirus species revealed highest similarity to MAdV-1, the representative of the murine adenovirus A species. However, substantial differences were found in the E1, E3, and E4 genomic regions. The phylogenetic distance of MAdV-3 amino acid sequences for pVIII, protease, polymerase, and hexon from MAdV-1 is markedly higher than 0.1 exchange per position, and, based on our cross-neutralization experiments, MAdV-3 and MAdV-1 can be regarded as different serotypes. Therefore, we propose to classify MAdV-3 as the first isolate of a novel adenovirus species, designated murine adenovirus C (MAdV-C). The novel MAdV-3 virus is not only genetically and serologically distinct from MAdV-1 but also shows a unique organ tropism in infected mice. In contrast to MAdV-1, the virus was not detectable in brain but predominantly infected heart tissue. Thus, infection of mice with cardiotropic MAdV-3 might be an interesting animal model of adenovirus-induced myocarditis.

* Corresponding author. Mailing address: Department of Molecular and Medical Virology, Ruhr-University Bochum, D-44780 Bochum, Germany. Phone: 49-234-3223189. Fax: 49-234-3214352. E-mail: klaus.ueberla{at}rub.de

{triangledown} Published ahead of print on 18 March 2009.

Journal of Virology, June 2009, p. 5749-5759, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.02281-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



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