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Journal of Virology, June 2009, p. 5574-5580, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.00001-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Regulation of Episomal Gene Expression by KRAB/KAP1-Mediated Histone Modifications{triangledown}

Isabelle Barde,1,2 Elisa Laurenti,1,{dagger} Sonia Verp,1,2 Anna Claire Groner,1,2 Christopher Towne,1 Viviane Padrun,1 Patrick Aebischer,1 Andreas Trumpp,1,{ddagger} and Didier Trono1,2*

School of Life Sciences,1 "Frontiers in Genetics" National Center for Competence in Research, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland2

Received 1 January 2009/ Accepted 6 March 2009

KAP1 is an essential cofactor of KRAB zinc finger proteins, a family of vertebrate-specific epigenetic repressors of largely unknown functions encoded in the hundreds by the mouse and human genomes. So far, KRAB/KAP1-mediated gene regulation has been studied within the environment of chromosomal DNA. Here we demonstrate that KRAB/KAP1 regulation is fully functional within the context of episomal DNA, such as adeno-associated viral and nonintegrated lentiviral vectors, and is correlated with histone modifications typically associated with this epigenetic regulator.

* Corresponding author. Mailing address: Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, EPFL SV-DO, Building SV, Station 17, CH-1015 Lausanne, Switzerland. Phone: 41 21 693 1751. Fax: 41 21 693 1635. E-mail: didier.trono{at}epfl.ch

{triangledown} Published ahead of print on 11 March 2009.

{dagger} Present address: Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, 1066 Epalinges, Switzerland.

{ddagger} Present address: Division of Cell Biology, German Cancer Research Center (DKFZ), and Heidelberg Institute for Stem Cell Technologies and Experimental Medicine, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.

Journal of Virology, June 2009, p. 5574-5580, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.00001-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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