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Journal of Virology, June 2009, p. 5535-5543, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.00393-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Plant Virus Cell-to-Cell Movement Is Not Dependent on the Transmembrane Disposition of Its Movement Protein{triangledown} ,{dagger}

Luis Martínez-Gil,1 Jesús A. Sánchez-Navarro,2 Antonio Cruz,3 Vicente Pallás,2 Jesús Pérez-Gil,3 and Ismael Mingarro1*

Departament de Bioquímica i Biologia Molecular, Universitat de València, E-46 100 Burjassot, Spain,1 Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, E-46022 Valencia, Spain,2 Departamento de Bioquímica y Biología Molecular I, Universidad Complutense Madrid, E-28040 Madrid, Spain3

Received 23 February 2009/ Accepted 17 March 2009

The cell-to-cell transport of plant viruses depends on one or more virus-encoded movement proteins (MPs). Some MPs are integral membrane proteins that interact with the membrane of the endoplasmic reticulum, but a detailed understanding of the interaction between MPs and biological membranes has been lacking. The cell-to-cell movement of the Prunus necrotic ringspot virus (PNRSV) is facilitated by a single MP of the 30K superfamily. Here, using a myriad of biochemical and biophysical approaches, we show that the PNRSV MP contains only one hydrophobic region (HR) that interacts with the membrane interface, as opposed to being a transmembrane protein. We also show that a proline residue located in the middle of the HR constrains the structural conformation of this region at the membrane interface, and its replacement precludes virus movement.

* Corresponding author. Mailing address: Departament de Bioquímica i Biologia Molecular, Universitat de València, E-46 100 Burjassot, Spain. Phone: 34-963543796. Fax: 34-963544635. E-mail: Ismael.Mingarro{at}uv.es

{triangledown} Published ahead of print on 25 March 2009.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, June 2009, p. 5535-5543, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.00393-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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