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Journal of Virology, May 2009, p. 5282-5288, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.02485-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Suppression of Host Gene Expression by nsp1 Proteins of Group 2 Bat Coronaviruses {triangledown}

Yukinobu Tohya,1,2 Krishna Narayanan,1 Wataru Kamitani,1 Cheng Huang,1 Kumari Lokugamage,1 and Shinji Makino1*

Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1019,1 Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan2

Received 3 December 2008/ Accepted 19 February 2009

nsp1 protein of severe acute respiratory syndrome coronavirus (SARS-CoV), a group 2b CoV, suppresses host gene expression by promoting host mRNA degradation and translation inhibition. The present study analyzed the activities of nsp1 proteins from the group 2 bat CoV strains Rm1, 133, and HKU9-1, belonging to groups 2b, 2c, and 2d, respectively. The host mRNA degradation and translational suppression activities of nsp1 of SARS-CoV and Rm1 nsp1 were similar and stronger than the activities of the nsp1 proteins of 133 and HKU9-1. Rm1 nsp1 expression in trans strongly inhibited the induction of type I interferon (IFN-I) and IFN-stimulated genes in cells infected with an IFN-inducing SARS-CoV mutant, while 133 and HKU9-1 nsp1 proteins had relatively moderate IFN-inhibitory activities. The results of our studies suggested a conserved function among nsp1 proteins of SARS-CoV and group 2 bat CoVs.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019. Phone: (409) 772-2323. Fax: (409) 772-5065. E-mail: shmakino{at}utmb.edu

{triangledown} Published ahead of print on 4 March 2009.

Journal of Virology, May 2009, p. 5282-5288, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.02485-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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