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Journal of Virology, May 2009, p. 5278-5281, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.00063-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Selection of H5N1 Influenza Virus PB2 during Replication in Humans{triangledown}

Quynh Mai Le,1 Yuko Sakai-Tagawa,2 Makoto Ozawa,2,3 Mustumi Ito,2 and Yoshihiro Kawaoka2,3,4,5,6*

National Institute of Hygiene and Epidemiology, Hanoi, Vietnam,1 Division of Virology, Department of Microbiology and Immunology,2 International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan,4 ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama 332-0012, Japan,5 Division of Zoonosis, Department of Microbiology and Infectious Diseases, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan,6 Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, Madison, Wisconsin 537063

Received 11 January 2009/ Accepted 17 February 2009

Highly pathogenic H5N1 influenza viruses continue to cause concern, even though currently circulating strains are not efficiently transmitted among humans. For efficient transmission, amino acid changes in viral proteins may be required. Here, we examined the amino acids at positions 627 and 701 of the PB2 protein. A direct analysis of the viral RNAs of H5N1 viruses in patients revealed that these amino acids contribute to efficient virus propagation in the human upper respiratory tract. Viruses grown in culture or eggs did not always reflect those in patients. These results emphasize the importance of the direct analysis of original specimens.

* Corresponding author. Mailing address: Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. Phone: 81-03-5449-5310. Fax: 81-03-5449-5408. E-mail: kawaoka{at}ims.u-tokyo.ac.jp

{triangledown} Published ahead of print on 4 March 2009.

Journal of Virology, May 2009, p. 5278-5281, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.00063-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shinya, K., Makino, A., Ozawa, M., Kim, J. H., Sakai-Tagawa, Y., Ito, M., Le, Q. M., Kawaoka, Y. (2009). Ostrich Involvement in the Selection of H5N1 Influenza Virus Possessing Mammalian-Type Amino Acids in the PB2 Protein. J. Virol. 83: 13015-13018 [Abstract] [Full Text]  
  • Lycett, S. J., Ward, M. J., Lewis, F. I., Poon, A. F. Y., Kosakovsky Pond, S. L., Brown, A. J. L. (2009). Detection of Mammalian Virulence Determinants in Highly Pathogenic Avian Influenza H5N1 Viruses: Multivariate Analysis of Published Data. J. Virol. 83: 9901-9910 [Abstract] [Full Text]  


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