This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jones, K. S.
Right arrow Articles by Mahieux, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jones, K. S.
Right arrow Articles by Mahieux, R.

 Previous Article  |  Next Article 

Journal of Virology, May 2009, p. 5244-5255, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.02285-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Receptor Complex Associated with Human T-Cell Lymphotropic Virus Type 3 (HTLV-3) Env-Mediated Binding and Entry Is Distinct from, but Overlaps with, the Receptor Complexes of HTLV-1 and HTLV-2{triangledown}

Kathryn S. Jones,1,¶* Ying K. Huang,2 Sébastien A. Chevalier,3 Philippe V. Afonso,3 Cari Petrow-Sadowski,1 Daniel C. Bertolette,2 Antoine Gessain,3 Francis W. Ruscetti,2 and Renaud Mahieux3,4,¶*

Basic Science Program, SAIC-Frederick, Inc,1 Laboratory of Experimental Immunology, Cancer and Inflammation Program, Center for Cancer Research, NCI-Frederick, Frederick, Maryland,2 Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Département de Virologie, Institut Pasteur, Paris, France,3 The George Washington University Medical Center, Washington, DC4

Received 31 October 2008/ Accepted 5 March 2009

Little is known about the transmission or tropism of the newly discovered human retrovirus, human T-cell lymphotropic virus type 3 (HTLV-3). Here, we examine the entry requirements of HTLV-3 using independently expressed Env proteins. We observed that HTLV-3 surface glycoprotein (SU) binds efficiently to both activated CD4+ and CD8+ T cells. This contrasts with both HTLV-1 SU, which primarily binds to activated CD4+ T cells, and HTLV-2 SU, which primarily binds to activated CD8+ T cells. Binding studies with heparan sulfate proteoglycans (HSPGs) and neuropilin-1 (NRP-1), two molecules important for HTLV-1 entry, revealed that these molecules also enhance HTLV-3 SU binding. However, unlike HTLV-1 SU, HTLV-3 SU can bind efficiently in the absence of both HSPGs and NRP-1. Studies of entry performed with HTLV-3 Env-pseudotyped viruses together with SU binding studies revealed that, for HTLV-1, glucose transporter 1 (GLUT-1) functions at a postbinding step during HTLV-3 Env-mediated entry. Further studies revealed that HTLV-3 SU binds efficiently to naïve CD4+ T cells, which do not bind either HTLV-1 or HTLV-2 SU and do not express detectable levels of HSPGs, NRP-1, and GLUT-1. These results indicate that the complex of receptor molecules used by HTLV-3 to bind to primary T lymphocytes differs from that of both HTLV-1 and HTLV-2.

* Corresponding author: Mailing address for Kathryn Jones: Basic Science Program, SAIC-Frederick, National Cancer Institute-Frederick, Frederick, MD 21702-1201. Phone: (301) 846-5262. Fax: (301) 846-7034. E-mail: joneska{at}mail.nih.gov. Mailing address for Renaud Mahieux: Equipe Oncogenèse Rétrovirale, INSERM-U758 Virologie Humaine, ENS Lyon, IFR 128 Biosciences Lyon Gerland, 46, allée d'Italie, 69364 Lyon Cedex 07, France. Phone: 33 4 72 72 87 93. Fax: 33 4 75 75 81 37. E-mail: renaud.mahieux{at}ens-lyon.fr

{triangledown} Published ahead of print on 11 March 2009.

K.S.J. and R.M. contributed equally to this work.

Journal of Virology, May 2009, p. 5244-5255, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.02285-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»