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Journal of Virology, May 2009, p. 5156-5167, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.02678-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The 30-Amino-Acid Deletion in the Nsp2 of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Emerging in China Is Not Related to Its Virulence{triangledown}

Lei Zhou,{dagger} Jialong Zhang,{dagger} Jingwen Zeng, Shuoyan Yin, Yanhua Li, Linying Zheng, Xin Guo, Xinna Ge, and Hanchun Yang*

Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, People's Republic of China

Received 30 December 2008/ Accepted 17 February 2009

During the past 2 years, an atypical clinical outbreak, caused by a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) with a unique 30-amino-acid deletion in its Nsp2-coding region, was pandemic in China. In this study, we generated four full-length infectious cDNA clones: a clone of the highly virulent PRRSV strain JXwn06 (pWSK-JXwn), a clone of the low-virulence PRRSV strain HB-1/3.9 (pWSK-HB-1/3.9), a chimeric clone in which the Nsp2 region containing the 30-amino-acid deletion was replaced by the corresponding region of the low-virulence PRRSV strain HB-1/3.9 (pWSK-JXwn-HB1nsp2), and a mutated HB-1/3.9 clone with the same deletion in Nsp2 as JXwn06 (pWSK-HB1-ND30). We also investigated the pathogenicities of the rescued viruses (designated RvJXwn, RvJXwn-HB1nsp2, RvHB-1/3.9, and RvHB1-ND30, respectively) in specific-pathogen-free piglets in order to determine the role of the 30-amino-acid deletion in the virulence of the highly pathogenic PRRSV. All the rescued viruses could replicate stably in MARC-145 cells. Our findings indicated that RvJXwn-HB1nsp2 retained high virulence for piglets, like RvJXwn and the parental virus JXwn06, although the survival time of piglets infected with RvJXwn-HB1nsp2 was obviously prolonged. RvHB1-ND30 exhibited low virulence for piglets, like RvHB-1/3.9 and the parental virus HB-1/3.9. Therefore, we conclude that the 30-amino-acid deletion is not related to the virulence of the highly pathogenic PRRSV emerging in China.

* Corresponding author. Mailing address: College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China. Phone and fax: 86-10-6273 1296. E-mail: yanghanchun1{at}cau.edu.cn

{triangledown} Published ahead of print on 25 February 2009.

{dagger} L. Zhou and J. Zhang contributed equally to this work.

Journal of Virology, May 2009, p. 5156-5167, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.02678-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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