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Journal of Virology, May 2009, p. 4732-4748, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.00111-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

NF-B p50 Plays Distinct Roles in the Establishment and Control of Murine Gammaherpesvirus 68 Latency

Laurie T. Krug,^1,2, Christopher M. Collins,^1,2 Lisa M. Gargano,^1,2 and Samuel H. Speck^1,2*

Emory Vaccine Center,¹ Department of Microbiology and Immunology, Emory University School of Medicine Atlanta, Georgia 30322²

Received 16 January 2009/ Accepted 24 February 2009

NF-B signaling is critical to the survival and transformation of cells infected by the human gammaherpesviruses Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus. Here we have examined how elimination of the NF-B transcription factor p50 from mice affects the life cycle of murine gammaherpesvirus 68 (MHV68). Notably, mice lacking p50 in every cell type were unable to establish a sufficiently robust immune response to control MHV68 infection, leading to high levels of latently infected B cells detected in the spleen and persistent virus replication in the lungs. The latter correlated with very low levels of virus-specific immunoglobulin G (IgG) in the infected p50^–/– mice at day 48 postinfection. Because the confounding impact of the loss of p50 on the host response to MHV68 infection prevented a direct analysis of the role of this NF-B family member on MHV68 latency in B cells, we generated and infected mixed p50^+/+/p50^–/– bone marrow chimeric mice. We show that the chimeric mice were able to control acute virus replication and exhibited normal levels of virus-specific IgG at 3 months postinfection, indicating the induction of a normal host immune response to MHV68 infection. However, in p50^+/+/p50^–/– chimeric mice the p50^–/– B cells exhibited a significant defect compared to p50^+/+ B cells in supporting MHV68 latency. In addition to identifying a role for p50 in the establishment of latency, we determined that the absence of p50 in a subset of the hematopoietic compartment led to persistent virus replication in the lungs of the chimeric mice, providing evidence that p50 is required for controlling virus reactivation. Taken together, these data demonstrate that p50 is required for immune control by the host and has distinct tissue-dependent roles in the regulation of murine gammaherpesvirus latency during chronic infection.

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* Corresponding author. Mailing address: Department of Microbiology and Immunology, Emory University School of Medicine, 1462 Clifton Road, Suite 429, Atlanta, GA 30322. Phone: (404) 727-7665. Fax: (404) 712-9736. E-mail: sspeck{at}emory.edu

Published ahead of print on 4 March 2009.

Present address: Stony Brook University, Life Sciences Bldg., Room 130, Stony Brook, NY 11794.

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Journal of Virology, May 2009, p. 4732-4748, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.00111-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.