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Journal of Virology, May 2008, p. 4675-4679, Vol. 82, No. 9
0022-538X/08/$08.00+0     doi:10.1128/JVI.02445-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Primer Binding Site-Dependent Restriction of Murine Leukemia Virus Requires HP1 Binding by TRIM28{triangledown} ,{dagger}

Daniel Wolf,1 Florence Cammas,2 Régine Losson,2 and Stephen P. Goff1*

Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, New York 10032,1 Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, BP10142, 67404 Illkirch-Cedex, France2

Received 13 November 2007/ Accepted 9 February 2008

TRIM28 is a transcriptional corepressor which is required for primer binding site (PBS)-dependent restriction of murine leukemia virus (MLV) replication in embryonic stem and embryonic carcinoma (EC) cells. PBS-dependent restriction of MLV leads to transcriptional silencing of the integrated provirus and has been shown to correlate with TRIM28-mediated recruitment of HP1 to the silenced loci. Here we show, using a cell line with a point mutation in the HP1 binding domain of TRIM28, that interaction with HP1 is absolutely required for the PBS-dependent restriction of MLV in the F9 EC cell line.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, HHSC 1310, 701 West 168th Street, New York, NY 10032. Phone: (212) 305-3794. Fax: (212) 305-5106. E-mail: goff{at}cancercenter.columbia.edu

{triangledown} Published ahead of print on 20 February 2008.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, May 2008, p. 4675-4679, Vol. 82, No. 9
0022-538X/08/$08.00+0     doi:10.1128/JVI.02445-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wolf, D., Hug, K., Goff, S. P. (2008). TRIM28 mediates primer binding site-targeted silencing of Lys1,2 tRNA-utilizing retroviruses in embryonic cells. Proc. Natl. Acad. Sci. USA 105: 12521-12526 [Abstract] [Full Text]  
  • Sauvain, M.-O., Dorr, A. P., Stevenson, B., Quazzola, A., Naef, F., Wiznerowicz, M., Schutz, F., Jongeneel, V., Duboule, D., Spitz, F., Trono, D. (2008). Genotypic Features of Lentivirus Transgenic Mice. J. Virol. 82: 7111-7119 [Abstract] [Full Text]  


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