JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JVI.02141-07v1
82/9/4413    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Ribet, D.
Right arrow Articles by Heidmann, T.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ribet, D.
Right arrow Articles by Heidmann, T.
Journal of Virology, May 2008, p. 4413-4419, Vol. 82, No. 9
0022-538X/08/$08.00+0     doi:10.1128/JVI.02141-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The GLN Family of Murine Endogenous Retroviruses Contains an Element Competent for Infectious Viral Particle Formation{triangledown}

David Ribet,1,2 Francis Harper,3 Cécile Esnault,1,2 Gérard Pierron,3 and Thierry Heidmann1,2*

Unité des Rétrovirus Endogènes et Eléments Rétroïdes des Eucaryotes Supérieurs, CNRS UMR 8122, Institut Gustave Roussy, Villejuif, F-94805 France,1 Université Paris-Sud, Orsay, F-91405 France,2 Laboratoire de Réplication de l'ADN et Ultrastructure du Noyau, FRE 2937, Institut André Lwoff, Villejuif, F-94801 France3

Received 28 September 2007/ Accepted 8 February 2008

Several families of endogenous retroviruses (ERVs) have been identified in the mouse genome, in several instances by in silico searches, but for many of them it remains to be determined whether there are elements that can still encode functional retroviral particles. Here, we identify, within the GLN family of highly reiterated ERVs, one, and only one, copy that encodes retroviral particles prone to infection of mouse cells. We show that its envelope protein confers an ecotropic host range and recognizes a receptor different from mCAT1 and mSMIT1, the two previously identified receptors for other ecotropic mouse retroviruses. Electron microscopy disclosed viral particle assembly and budding at the cell membrane, as well as release of mature particles into the extracellular space. These particles are closely related to murine leukemia virus (MLV) particles, with which they have most probably been confused in the past. This study, therefore, identifies a new class of infectious mouse ERVs belonging to the family Gammaretroviridae, with one family member still functional today. This family is in addition to the two MLV and mouse mammary tumor virus families of active mouse ERVs with an extracellular life cycle.

* Corresponding author. Mailing address: Unité des Rétrovirus Endogènes et Eléments Rétroïdes des Eucaryotes Supérieurs, CNRS UMR 8122, Institut Gustave Roussy, Villejuif, F-94805 France. Phone: 33/1-42-11-54-33. Fax: 33/1-42-11-53-42. E-mail: heidmann{at}igr.fr

{triangledown} Published ahead of print on 20 February 2008.

Journal of Virology, May 2008, p. 4413-4419, Vol. 82, No. 9
0022-538X/08/$08.00+0     doi:10.1128/JVI.02141-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2008 by the American Society for Microbiology. All rights reserved.