This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yao, Y.
Right arrow Articles by Nair, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yao, Y.
Right arrow Articles by Nair, V.

 Previous Article  |  Next Article 

Journal of Virology, April 2008, p. 4007-4015, Vol. 82, No. 8
0022-538X/08/$08.00+0     doi:10.1128/JVI.02659-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

MicroRNA Profile of Marek's Disease Virus-Transformed T-Cell Line MSB-1: Predominance of Virus-Encoded MicroRNAs{triangledown}

Yongxiu Yao,1 Yuguang Zhao,1 Hongtao Xu,1 Lorraine P. Smith,1 Charles H. Lawrie,2 Michael Watson,1 and Venugopal Nair1*

Division of Microbiology, Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom,1 LRF Molecular Haematology Unit, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford OX3 9DU, United Kingdom2

Received 14 December 2007/ Accepted 25 January 2008

Research over the last few years has demonstrated the increasing role of microRNAs (miRNAs) as major regulators of gene expression in diverse cellular processes and diseases. Several viruses, particularly herpesviruses, also use the miRNA pathway of gene regulation by encoding their own miRNAs. Marek's disease (MD) is a widespread lymphomatous neoplastic disease of poultry caused by the highly contagious Marek's disease virus type 1 (MDV-1). Recent studies using virus-infected chicken embryo fibroblasts have identified at least eight miRNAs that map to the RL/RS region of the MDV genome. Since MDV is a lymphotropic virus that induces T-cell lymphomas, analysis of the miRNA profile in T-cell lymphoma would be more relevant for examining their role in oncogenesis. We determined the viral and host miRNAs expressed in MSB-1, a lymphoblastoid cell line established from an MDV-induced lymphoma of the spleen. In this paper, we report the identification of 13 MDV-1-encoded miRNAs (12 by direct cloning and 1 by Northern blotting) from MSB-1 cells. These miRNAs, five of which are novel MDV-1 miRNAs, map to the Meq and latency-associated transcript regions of the MDV genome. Furthermore, we show that miRNAs encoded by MDV-1 and the coinfected MDV-2 accounted for >60% of the 5,099 sequences of the MSB-1 "miRNAome." Several chicken miRNAs, some of which are known to be associated with cancer, were also cloned from MSB-1 cells. High levels of expression of MDV-1-encoded miRNAs and potentially oncogenic host miRNAs suggest that miRNAs may have major roles in MDV pathogenesis and neoplastic transformation.

* Corresponding author. Mailing address: Division of Microbiology, Institute for Animal Health, Compton, Berkshire, United Kingdom RG20 7NN. Phone: 441635 577356. Fax: 441635 577263. E-mail: venu.gopal{at}bbsrc.ac.uk

{triangledown} Published ahead of print on 6 February 2008.

Journal of Virology, April 2008, p. 4007-4015, Vol. 82, No. 8
0022-538X/08/$08.00+0     doi:10.1128/JVI.02659-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yao, Y., Zhao, Y., Smith, L. P., Lawrie, C. H., Saunders, N. J., Watson, M., Nair, V. (2009). Differential expression of microRNAs in Marek's disease virus-transformed T-lymphoma cell lines. J. Gen. Virol. 90: 1551-1559 [Abstract] [Full Text]  
  • Yao, Y., Zhao, Y., Smith, L. P., Watson, M., Nair, V. (2009). Novel MicroRNAs (miRNAs) Encoded by Herpesvirus of Turkeys: Evidence of miRNA Evolution by Duplication. J. Virol. 83: 6969-6973 [Abstract] [Full Text]  
  • Umbach, J. L., Cullen, B. R. (2009). The role of RNAi and microRNAs in animal virus replication and antiviral immunity. Genes Dev. 23: 1151-1164 [Abstract] [Full Text]  
  • Lambeth, L. S., Yao, Y., Smith, L. P., Zhao, Y., Nair, V. (2009). MicroRNAs 221 and 222 target p27Kip1 in Marek's disease virus-transformed tumour cell line MSB-1. J. Gen. Virol. 90: 1164-1171 [Abstract] [Full Text]  
  • Ghosh, Z., Mallick, B., Chakrabarti, J. (2009). Cellular versus viral microRNAs in host-virus interaction. Nucleic Acids Res 37: 1035-1048 [Abstract] [Full Text]  
  • Zhao, Y., Yao, Y., Xu, H., Lambeth, L., Smith, L. P., Kgosana, L., Wang, X., Nair, V. (2009). A Functional MicroRNA-155 Ortholog Encoded by the Oncogenic Marek's Disease Virus. J. Virol. 83: 489-492 [Abstract] [Full Text]  
  • Morgan, R., Anderson, A., Bernberg, E., Kamboj, S., Huang, E., Lagasse, G., Isaacs, G., Parcells, M., Meyers, B. C., Green, P. J., Burnside, J. (2008). Sequence Conservation and Differential Expression of Marek's Disease Virus MicroRNAs. J. Virol. 82: 12213-12220 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»