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Journal of Virology, March 2008, p. 2590-2593, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.01956-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Relative Influence of the Adeno-Associated Virus (AAV) Type 2 p5 Element for Recombinant AAV Vector Site-Specific Integration{triangledown}

Mickaël Guilbaud,1,2 Gilliane Chadeuf,1 Fabio Avolio,3 Achille François,1 Philippe Moullier,1,2 Alessandra Recchia,3,4 and Anna Salvetti1,5*

INSERM, U649, Nantes F-44000, France,1 EFS des Pays de la Loire, Nantes F-4400, France,2 Istituto Scientifico H. San Raffaele, Gene Therapy Program, Milano, Italy,3 University of Modena and Reggio Emilia, Department of Biomedical Sciences, Modena, Italy,4 INSERM, U758, Lyon F-69007, France5

Received 6 September 2007/ Accepted 12 December 2007

The p5 promoter region of the adeno-associated virus type 2 (AAV-2) rep gene has been described as essential for Rep-mediated site-specific integration (RMSSI) of plasmid sequences in human chromosome 19. We report here that insertion of a full-length or minimal p5 element between the viral inverted terminal repeats does not significantly increase RMSSI of a recombinant AAV (rAAV) vector after infection of growth-arrested or proliferating human cells. This result suggests that the p5 element may not improve RMSSI of rAAV vectors in vivo.

* Corresponding author. Mailing address: INSERM U758, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, F-69007 Lyon, France. Phone: 33 472728401. Fax: 33 472728137. E-mail: anna.salvetti{at}ens-lyon.fr

{triangledown} Published ahead of print on 19 December 2007.

Journal of Virology, March 2008, p. 2590-2593, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.01956-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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