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Journal of Virology, March 2008, p. 2575-2579, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.00962-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Heat Shock Perturbs TRIM5{alpha} Restriction of Human Immunodeficiency Virus Type 1{triangledown}

Jenny L. Anderson, Edward M. Campbell, Anna Figueiredo, and Thomas J. Hope*

Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611

Received 3 May 2007/ Accepted 28 November 2007

TRIM5{alpha} restriction factors protect target cells from retroviruses by blocking infection prior to the accumulation of viral reverse transcription (RT) products. Here, we demonstrate that heat shock perturbed owl monkey TRIMCyp and rhesus TRIM5{alpha}-mediated restriction of human immunodeficiency virus type 1 (HIV-1) late RT products and 2-long terminal repeat circles. Heat shock partially rescued HIV-1 infection from TRIMCyp restriction, and this rescue became more profound when combined with the presence of the proteasome inhibitor MG132. This indicates that viral RT products rescued from restriction by either heat shock treatment or the presence of MG132 are on a productive pathway, supporting a model in which TRIM5{alpha} proteins restrict retroviruses in multiple phases that are differentially sensitive to heat shock and proteasome inhibitors.

* Corresponding author. Mailing address: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Ward 8-140, 303 East Chicago Avenue, Chicago, IL 60611. Phone: (312) 503-1360. Fax: (312) 503-7912. E-mail: thope{at}northwestern.edu

{triangledown} Published ahead of print on 12 December 2007.

Journal of Virology, March 2008, p. 2575-2579, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.00962-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Richardson, M. W., Carroll, R. G., Stremlau, M., Korokhov, N., Humeau, L. M., Silvestri, G., Sodroski, J., Riley, J. L. (2008). Mode of Transmission Affects the Sensitivity of Human Immunodeficiency Virus Type 1 to Restriction by Rhesus TRIM5{alpha}. J. Virol. 82: 11117-11128 [Abstract] [Full Text]  


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