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Journal of Virology, March 2008, p. 2555-2559, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.01853-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Jaagsiekte Sheep Retrovirus Utilizes a pH-Dependent Endocytosis Pathway for Entry

Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada,¹ Department of Molecular Sciences, University of Tennessee Health Sciences Center, Memphis, Tennessee 38163²

Received 22 August 2007/ Accepted 13 December 2007

Using Moloney murine leukemia virus pseudovirions bearing the envelope protein of Jaagsiekte sheep retrovirus (JSRV), we report here that entry was weakly inhibited by lysosomotropic agents but was profoundly blocked by bafilomycin A1 (BafA1). Kinetics studies revealed that JSRV entry is a slow process and was substantially blocked by a dominant-negative mutant of dynamin. Interestingly, a low-pH pulse overcame the BafA1 block to JSRV infection, although this occurred only if virus-bound cells were preincubated at 37°C, consistent with a very early entry event such as endocytosis being required before the low-pH-dependent step occurs. Moreover, JSRV pseudovirions were resistant to low-pH inactivation. Altogether, this study reveals that JSRV utilizes a pH-dependent, dynamin-associated endocytosis pathway for entry that differs from the classical pH-dependent entry pathway of vesicular stomatitis virus.

Published ahead of print on 19 December 2007.

This article has been cited by other articles:

• Cote, M., Zheng, Y.-M., Albritton, L. M., Liu, S.-L. (2008). Fusogenicity of Jaagsiekte Sheep Retrovirus Envelope Protein Is Dependent on Low pH and Is Enhanced by Cytoplasmic Tail Truncations. J. Virol. 82: 2543-2554 [Abstract] [Full Text]