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Journal of Virology, March 2008, p. 2324-2329, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.01930-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Global Transcriptional Responses of Pseudomonas aeruginosa to Phage PRR1 Infection

Janne J. Ravantti, Tanja M. Ruokoranta,^, A. Marika Alapuranen, and Dennis H. Bamford^*

Department of Biological and Environmental Sciences and Institute of Biotechnology, Viikki Biocenter, P.O. Box 56, 00014 University of Helsinki, Finland

Received 4 September 2007/ Accepted 3 December 2007

The infectious cycles of viruses are known to cause dramatic changes to host cell function. The development of microarray technology has provided means to monitor host cell responses to viral infection at the level of global changes in mRNA levels. We have applied this methodology to investigate gene expression changes caused by a small, icosahedral, single-stranded-RNA phage, PRR1 (a member of the Leviviridae family), on its host, Pseudomonas aeruginosa, at different times during its growth cycle. Viral infection in this system resulted in changes in expression levels of <4% of P. aeruginosa genes. Interestingly, the number of genes affected by viral infection was significantly lower than the number of genes affected by changes in growth conditions during the experiment. Compared with a similar study that focused on the complex, double-stranded-DNA bacterial virus PRD1, it was evident that there were no universal responses to viral infection. However, in both cases, translation was affected in infected cells.

Published ahead of print on 12 December 2007.

J.J.R. and T.M.R. contributed equally to this work.

Present address: Vactech Oy, Biokatu 8, 33520 Tampere, Finland.

Present address: Roal Oy, P.O. Box 57, 05201 Rajamäki, Finland.