This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Katpally, U.
Right arrow Articles by Smith, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Katpally, U.
Right arrow Articles by Smith, T. J.

 Previous Article  |  Next Article 

Journal of Virology, March 2008, p. 2079-2088, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.02200-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Structure of Antibody-Neutralized Murine Norovirus and Unexpected Differences from Viruslike Particles{triangledown}

Umesh Katpally,1 Christiane E. Wobus,2,{dagger} Kelly Dryden,3 Herbert W. Virgin IV,2 and Thomas J. Smith1*

Donald Danforth Plant Science Center, Saint Louis, Missouri 63132,1 Department of Pathology and Immunology and Department of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri 63110,2 Department of Cell Biology, Scripps Research Institute, La Jolla, CA 920373

Received 8 October 2007/ Accepted 7 December 2007

Noroviruses (family Caliciviridae) are the major cause of epidemic nonbacterial gastroenteritis in humans, but the mechanism of antibody neutralization is unknown and no structure of an infectious virion has been reported. Murine norovirus (MNV) is the only norovirus that can be grown in tissue culture, studied in an animal model, and reverse engineered via an infectious clone and to which neutralizing antibodies have been isolated. Presented here are the cryoelectron microscopy structures of an MNV virion and the virion in complex with neutralizing Fab fragments. The most striking differences between MNV and previous calicivirus structures are that the protruding domain is lifted off the shell domain by ~16Å and rotated ~40° in a clockwise fashion and forms new interactions at the P1 base that create a cagelike structure engulfing the shell domains. Neutralizing Fab fragments cover the outer surface of each copy of the capsid protein P2 domains without causing any apparent conformational changes. These unique features of MNV suggest that at least some caliciviruses undergo a capsid maturation process akin to that observed with other plant and bacterial viruses.

* Corresponding author. Mailing address: Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO 63132. Phone: (314) 587-1451. Fax: (314) 587-1551. E-mail: tsmith{at}danforthcenter.org

{triangledown} Published ahead of print on 19 December 2007.

{dagger} Present address: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620.

Journal of Virology, March 2008, p. 2079-2088, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.02200-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hafenstein, S., Bowman, V. D., Sun, T., Nelson, C. D. S., Palermo, L. M., Chipman, P. R., Battisti, A. J., Parrish, C. R., Rossmann, M. G. (2009). Structural Comparison of Different Antibodies Interacting with Parvovirus Capsids. J. Virol. 83: 5556-5566 [Abstract] [Full Text]  
  • Bailey, D., Thackray, L. B., Goodfellow, I. G. (2008). A Single Amino Acid Substitution in the Murine Norovirus Capsid Protein Is Sufficient for Attenuation In Vivo. J. Virol. 82: 7725-7728 [Abstract] [Full Text]  
  • Chachu, K. A., Strong, D. W., LoBue, A. D., Wobus, C. E., Baric, R. S., Virgin, H. W. IV (2008). Antibody Is Critical for the Clearance of Murine Norovirus Infection. J. Virol. 82: 6610-6617 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»