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Journal of Virology, February 2008, p. 2033-2037, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.01774-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Replication of Coxsackievirus B3 in Primary Cell Cultures Generates Novel Viral Genome Deletions{triangledown}

K.-S. Kim,* N. M. Chapman, and S. Tracy

Enterovirus Research Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, Nebraska 68198-6495

Received 13 August 2007/ Accepted 24 November 2007

Coxsackievirus B3 (CVB3) generates 5'-terminally deleted genomes (TDs) during replication in murine hearts. We show here that CVB3 populations with TDs can also be generated within two to three passages of CVB3 in primary, but not immortalized, cell cultures. Deletions of less than 49 nucleotides increase in size during passage, while 5' TDs of 49 nucleotides appear to be the maximum deletion size. The cellular environment of contact-inhibited primary cell cultures or the myocardium in vivo is sufficient for the selection of 5' TDs over undeleted genomes.

* Corresponding author. Mailing address: Department of Pathology and Microbiology, University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, NE 68198-6495. Phone: (402) 559-7667. Fax: (402) 559-4077. E-mail: kkim{at}unmc.edu

{triangledown} Published ahead of print on 5 December 2007.

Journal of Virology, February 2008, p. 2033-2037, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.01774-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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