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Journal of Virology, February 2008, p. 2028-2032, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.02065-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

H5-Type Influenza Virus Hemagglutinin Is Functionally Recognized by the Natural Killer-Activating Receptor NKp44{triangledown}

Joanna W. Ho,1 Oren Hershkovitz,2 Malik Peiris,1,3 Alon Zilka,2 Ahuva Bar-Ilan,2 Beatrice Nal,1 Kid Chu,1 Mateusz Kudelko,1 Yiu Wing Kam,1 Hagit Achdout,4 Michal Mandelboim,5 Ralf Altmeyer,1,6 Ofer Mandelboim,4 Roberto Bruzzone,1 and Angel Porgador2*

Hong Kong University-Pasteur Research Center, Hong Kong SAR, China,1 Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences and Cancer Research Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel,2 Department of Microbiology, Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China,3 Lautenberg Center for General and Tumor Immunology, Hadassah Medical School, Hebrew University, Jerusalem, Israel,4 Central Virology Laboratory, Public Health Services, Ministry of Health, Sheba Medical Center, Tel-Hashomer, Israel,5 CombinatoRx-Singapore Pte. Ltd., 11 Biopolis Way, Singapore, Republic of Singapore6

Received 18 September 2007/ Accepted 26 November 2007

Antiviral immune defenses involve natural killer (NK) cells. We previously showed that the NK-activating receptor NKp44 is involved in the functional recognition of H1-type influenza virus strains by NK cells. In the present study, we investigated the interaction of NKp44 and the hemagglutinin of a primary influenza virus H5N1 isolate. Here we show that recombinant NKp44 recognizes H5-expressing cells and specifically interacts with soluble H5 hemagglutinin. H5-pseudotyped lentiviral particles bind to NK cells expressing NKp44. Following interaction with target cells expressing H5, pseudotyped lentiviral particles, or membrane-associated H5, NK cells show NKp44-mediated induced activity. These findings indicate that NKp44-H5 interactions induce functional NK activation.

* Corresponding author. Mailing address: Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel. Phone: 972-8-647-7283. Fax: 972-8-647-2574. E-mail: angel{at}bgu.ac.il

{triangledown} Published ahead of print on 12 December 2007.

Journal of Virology, February 2008, p. 2028-2032, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.02065-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Hershkovitz, O., Rosental, B., Rosenberg, L. A., Navarro-Sanchez, M. E., Jivov, S., Zilka, A., Gershoni-Yahalom, O., Brient-Litzler, E., Bedouelle, H., Ho, J. W., Campbell, K. S., Rager-Zisman, B., Despres, P., Porgador, A. (2009). NKp44 Receptor Mediates Interaction of the Envelope Glycoproteins from the West Nile and Dengue Viruses with NK Cells. J. Immunol. 183: 2610-2621 [Abstract] [Full Text]  


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