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Journal of Virology, February 2008, p. 2025-2027, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.02278-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rescue of Maturation-Defective Flock House Virus Infectivity with Noninfectious, Mature, Viruslike Particles{triangledown}

Hanna E. Walukiewicz,1,{dagger} Manidipa Banerjee,2 Anette Schneemann,2 and John E. Johnson1,2*

Department of Chemistry and Biochemistry, University of California—San Diego,1 Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 920372

Received 20 October 2007/ Accepted 29 November 2007

The infectivity of flock house virus (FHV) requires autocatalytic maturation cleavage of the capsid protein at residue 363, liberating the C-terminal 44-residue {gamma} peptides, which remain associated with the particle. In vitro studies previously demonstrated that the amphipathic, helical portion (amino acids 364 to 385) of {gamma} is membrane active, suggesting a role for {gamma} in RNA membrane translocation during infection. Here we show that the infectivity of a maturation-defective mutant of FHV can be restored by viruslike particles that lack the genome but undergo maturation cleavage. We propose that the colocalization of the two defective particle types in an entry compartment allows the restoration of infectivity by {gamma}.

* Corresponding author. Mailing address: Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd. Mail MB-31, La Jolla, CA 92037. Phone: (858) 784-9705. Fax: (858) 784-8660. E-mail: jackj{at}scripps.edu

{triangledown} Published ahead of print on 12 December 2007.

{dagger} Present address: Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801.

Journal of Virology, February 2008, p. 2025-2027, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.02278-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Banerjee, M., Khayat, R., Walukiewicz, H. E., Odegard, A. L., Schneemann, A., Johnson, J. E. (2009). Dissecting the Functional Domains of a Nonenveloped Virus Membrane Penetration Peptide. J. Virol. 83: 6929-6933 [Abstract] [Full Text]  


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