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Journal of Virology, February 2008, p. 1851-1859, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.02339-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Genome Organization and Reverse Genetic Analysis of a Type I Feline Coronavirus{triangledown}

Gergely Tekes,1 Regina Hofmann-Lehmann,2 Iris Stallkamp,1 Volker Thiel,3* and Heinz-Jürgen Thiel1*

Institut für Virologie, Justus-Liebig-Universität Giessen, Giessen, Germany,1 Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland,2 Research Department, Kantonal Hospital St. Gallen, St. Gallen, Switzerland3

Received 29 October 2007/ Accepted 28 November 2007

In this study we report the complete sequence and genome organization of the serotype I feline coronavirus (FCoV) strain Black. Furthermore, a reverse genetic system was established for this FCoV strain by cloning a full-length cDNA copy into vaccinia virus. This clone served as basis for the generation of recombinant FCoV (recFCoV) that was shown to bear the same features in vitro as the parental FCoV. Using this system, accessory 3abc genes in the FCoV genome were replaced by green fluorescent protein (recFCoV-GFP) and Renilla luciferase genes (recFCoV-RL). In addition, we showed that feline CD14+ blood monocytes and dendritic cells can be easily detected after infection with recFCoV-GFP. Thus, our established reverse genetic system provides a suitable tool to study the molecular biology of serotype I FCoV.

* Corresponding author. Mailing address for Volker Thiel: Kantonal Hospital St. Gallen, Research Department, 9007 St. Gallen, Switzerland. Phone: 41 71 4942843. Fax: 41 71 4946321. E-mail: volker.thiel{at}kssg.ch. Mailing address for Heinz-Jürgen Thiel: Justus-Liebig-Universität Giessen, Institut für Virologie, 35392 Giessen, Germany. Phone: 49 641 9938350. Fax: 49 641 9938359. E-mail: heinz-juergen.thiel{at}vetmed.uni-giessen.de

{triangledown} Published ahead of print on 12 December 2007.

Journal of Virology, February 2008, p. 1851-1859, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.02339-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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