This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, W. L.
Right arrow Articles by Chen, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, W. L.
Right arrow Articles by Chen, H.

 Previous Article  |  Next Article 

Journal of Virology, February 2008, p. 1798-1807, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.02256-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Antigenic Profile of Avian H5N1 Viruses in Asia from 2002 to 2007{triangledown}

Wai Lan Wu,1,{dagger} Yixin Chen,3,4,{dagger} Pui Wang,1 Wenjun Song,1 Siu-Ying Lau,1 Jane M. Rayner,1 Gavin J. D. Smith,1 Robert G. Webster,5 J. S. Malik Peiris,1 Tianwei Lin,1,4 Ningshao Xia,3,4 Yi Guan,1,2* and Honglin Chen1,2*

State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology and the Research Center of Infection and Immunology, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, People's Republic of China,1 International Institute of Infection and Immunity, Shantou University, Shantou, People's Republic of China,2 National Institute of Diagnostics and Vaccine Development in Infectious Diseases,3 School of Life Sciences, Xiamen University, Xiamen, People's Republic of China,4 Virology Division, Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 381055

Received 18 October 2007/ Accepted 26 November 2007

Antigenic profiles of post-2002 H5N1 viruses representing major genetic clades and various geographic sources were investigated using a panel of 17 monoclonal antibodies raised from five H5N1 strains. Four antigenic groups from seven clades of H5N1 virus were distinguished and characterized based on their cross-reactivity to the monoclonal antibodies in hemagglutination inhibition and cell-based neutralization assays. Genetic polymorphisms associated with the variation of antigenicity of H5N1 strains were identified and further verified in antigenic analysis with recombinant H5N1 viruses carrying specific mutations in the hemagglutinin protein. Modification of some of these genetic variations produced marked improvement to the immunogenicity and cross-reactivity of H5N1 strains in assays utilizing monoclonal antibodies and ferret antisera raised against clade 1 and 2 H5N1 viruses, suggesting that these sites represent antigenically significant amino acids. These results provide a comprehensive antigenic profile for H5N1 virus strains circulating in recent years and will facilitate the recognition of emerging antigenic variants of H5N1 virus and aid in the selection of vaccine strains.

* Corresponding author. Mailing address: State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, People's Republic of China. Phone: 852 28199830. Fax: 852 28551241. E-mail for H. Chen: hlchen{at}hkucc.hku.hk. E-mail for Y. Guan: yguan{at}hkucc.hku.hk

{triangledown} Published ahead of print on 12 December 2007.

{dagger} W.L.W. and Y.C. contributed equally to this study.

Journal of Virology, February 2008, p. 1798-1807, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.02256-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cusick, M. F., Wang, S., Eckels, D. D. (2009). In Vitro Responses to Avian Influenza H5 by Human CD4 T Cells. J. Immunol. 183: 6432-6441 [Abstract] [Full Text]  
  • Lycett, S. J., Ward, M. J., Lewis, F. I., Poon, A. F. Y., Kosakovsky Pond, S. L., Brown, A. J. L. (2009). Detection of Mammalian Virulence Determinants in Highly Pathogenic Avian Influenza H5N1 Viruses: Multivariate Analysis of Published Data. J. Virol. 83: 9901-9910 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»