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Journal of Virology, February 2008, p. 1638-1646, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.01917-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Noncanonical TATA Sequence in the UL44 Late Promoter of Human Cytomegalovirus Is Required for the Accumulation of Late Viral Transcripts{triangledown}

Hiroki Isomura,1* Mark F. Stinski,2 Ayumi Kudoh,1 Takayuki Murata,1 Sanae Nakayama,1 Yoshitaka Sato,1 Satoko Iwahori,1 and Tatsuya Tsurumi1

Division of Virology, Aichi Cancer Center Research Institute, 1-1, Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan,1 Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, Iowa 522422

Received 2 September 2007/ Accepted 19 November 2007

During productive infection, human cytomegalovirus (HCMV) UL44 transcription initiates at three distinct start sites that are differentially regulated. Two of the start sites, the distal and the proximal, are active at early times, whereas the middle start site is active only at late times after infection. The UL44 early viral gene product is essential for viral DNA synthesis. The UL44 gene product from the late viral promoter affects primarily viral gene expression at late times after infection rather than viral DNA synthesis (H. Isomura, M. F. Stinski, A. Kudoh, S. Nakayama, S. Iwahori, Y. Sato, and T. Tsurumi, J. Virol. 81:6197, 2007). The UL44 early viral promoters have a canonical TATA sequence, "TATAA." In contrast, the UL44 late viral promoter has a noncanonical TATA sequence. Using recombinant viruses, we found that the noncanonical TATA sequence is required for the accumulation of late viral transcripts. The GC boxes that surround the middle TATA element did not affect the kinetics or the start site of UL44 late transcription. Replacement of the distal TATA element with a noncanonical TATA sequence did not affect the kinetics of transcription or the transcription start site, but it did induce an alternative transcript at late times after infection. The data indicate that a noncanonical TATA box is used at late times after HCMV infection.

* Corresponding author. Mailing address: Division of Virology, Aichi Cancer Center Research Institute, 1-1, Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan. Phone: 81-52-762-6111, ext. 7032. Fax: 81-52-763-5233. E-mail: hisomura{at}aichi-cc.jp

{triangledown} Published ahead of print on 5 December 2007.

Journal of Virology, February 2008, p. 1638-1646, Vol. 82, No. 4
0022-538X/08/$08.00+0     doi:10.1128/JVI.01917-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakayama, S., Murata, T., Murayama, K., Yasui, Y., Sato, Y., Kudoh, A., Iwahori, S., Isomura, H., Kanda, T., Tsurumi, T. (2009). Epstein-Barr Virus Polymerase Processivity Factor Enhances BALF2 Promoter Transcription as a Coactivator for the BZLF1 Immediate-Early Protein. J. Biol. Chem. 284: 21557-21568 [Abstract] [Full Text]  


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