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Establishment of Canine RNA Polymerase I-Driven Reverse Genetics for Influenza A Virus: Its Application for H5N1 Vaccine Production

Division of Virology, Department of Microbiology and Immunology,¹ International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan,² Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Saitama, Japan,³ Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin⁴

Received 27 August 2007/ Accepted 12 November 2007

In the event of a new influenza pandemic, vaccines whose antigenicities match those of circulating strains must be rapidly produced. Here, we established an alternative reverse genetics system for influenza virus using the canine polymerase I (PolI) promoter sequence that works efficiently in the Madin-Darby canine kidney cell line, a cell line approved for human vaccine production. Using this system, we were able to generate H5N1 vaccine seed viruses more efficiently than can be achieved with the current system that uses the human PolI promoter in African green monkey Vero cells, thus improving pandemic vaccine production.

Published ahead of print on 28 November 2007.