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 Previous Article

Journal of Virology, December 2008, p. 12598-12601, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01732-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

VP26 of White Spot Syndrome Virus Functions as a Linker Protein between the Envelope and Nucleocapsid of Virions by Binding with VP51{triangledown}

Qiang Wan,1 Limei Xu,2 and Feng Yang2,3*

School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China,1 Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, People's Republic of China,2 College of Oceanography and Environmental Science of Xiamen University, Xiamen 361005, People's Republic of China3

Received 15 August 2008/ Accepted 25 September 2008

The envelopment of the nucleocapsid is an important step in white spot syndrome virus (WSSV) assembly. Previous studies showed that VP26, a major envelope protein of WSSV, can interact with viral nucleocapsid. In this study, using the biotin label transfer technique, we found that the biotin label was transferred from Bio-rVP26 to the viral capsid protein VP51 or from Bio-MBP-VP51 to VP26. Far-Western analyses provided further evidence for direct interaction between VP26 and VP51. Therefore, we conclude that VP26 functions as a matrix-like linker protein between the viral envelope and nucleocapsid, which suggests that VP26 is a key factor in the envelopment of WSSV virion.

* Corresponding author. Mailing address: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA. 178 Daxue Rd., Xiamen 361005, P. R. China. Phone: 86-0592-2195274. Fax: 86-0592-2085376. E-mail: mbiotech{at}public.xm.fj.cn

{triangledown} Published ahead of print on 8 October 2008.

Journal of Virology, December 2008, p. 12598-12601, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01732-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhou, Q., Xu, L., Li, H., Qi, Y.-P., Yang, F. (2009). Four Major Envelope Proteins of White Spot Syndrome Virus Bind To Form a Complex. J. Virol. 83: 4709-4712 [Abstract] [Full Text]  


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