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Journal of Virology, December 2008, p. 12585-12588, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01726-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of Gammaretroviruses Constitutively Released from Cell Lines Used for Human Immunodeficiency Virus Research{triangledown}

Yasuhiro Takeuchi,1 Myra O. McClure,2 and Massimo Pizzato2*

Wohl Virion Centre, Division of Infection and Immunity, University College London, London W1T 4JF, United Kingdom,1 Department of Infectious Diseases, Division of Medicine, Imperial College London, London W2 1PG, United Kingdom2

Received 14 August 2008/ Accepted 29 September 2008

Three human cell lines used in human immunodeficiency virus research were found to be contaminated with previously undetected retroviruses. On the bases of partial nucleotide sequence, capsid protein antigenicity, vector mobilization, and receptor usage studies, these contaminants were shown to be replication competent and to belong to the Gammaretrovirus genus. While the TZM-bl cells harbor ecotropic murine leukemia virus (MLV), Jurkat J6 cells were found to release xenotropic MLV and the A3.01/F7 cells to produce gibbon ape leukemia virus. These findings highlight the importance of routine testing of cell lines for retrovirus contamination to prevent potential experimental artifacts and allow correct biohazard assessment.

* Corresponding author. Mailing address: Imperial College London, St. Mary's Campus, Norfolk Place, London W2 1PG, United Kingdom. Phone: 44(0)20 7594 3918. Fax: 44(0)20 7594 3906. E-mail: m.pizzato{at}imperial.ac.uk

{triangledown} Published ahead of print on 8 October 2008.

Journal of Virology, December 2008, p. 12585-12588, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01726-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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