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Journal of Virology, December 2008, p. 12565-12568, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01631-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Heparan Sulfate-Independent Cell Binding and Infection with Furin-Precleaved Papillomavirus Capsids{triangledown}

Patricia M. Day,* Douglas R. Lowy, and John T. Schiller

Laboratory of Cellular Oncology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

Received 30 July 2008/ Accepted 23 September 2008

Papillomavirus infection normally involves virion binding to cell surface heparan sulfate proteoglycans (HSPGs). However, we found that human papillomavirus type 16 pseudovirions efficiently bound and infected cells lacking HSPGs if their L2 capsid protein was precleaved by furin, a cellular protease required for infection. The inability of pseudovirions to efficiently bind and infect cultured primary keratinocytes was also overcome by furin precleavage, suggesting that the defect involves altered HSPG modification. We conclude that the primary function of HSPG binding is to enable cell surface furin cleavage of L2 and that binding to a distinct cell surface receptor(s) is a subsequent step of papillomavirus infection.

* Corresponding author. Mailing address: Laboratory of Cellular Oncology, National Cancer Institute, National Institutes of Health, Bethesda, MD. Phone: (301) 594-6945. Fax: (301) 480-5322. E-mail: pmd{at}nih.gov

{triangledown} Published ahead of print on 1 October 2008.

Journal of Virology, December 2008, p. 12565-12568, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01631-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Johnson, K. M., Kines, R. C., Roberts, J. N., Lowy, D. R., Schiller, J. T., Day, P. M. (2009). Role of Heparan Sulfate in Attachment to and Infection of the Murine Female Genital Tract by Human Papillomavirus. J. Virol. 83: 2067-2074 [Abstract] [Full Text]  


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