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Journal of Virology, December 2008, p. 12555-12564, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01238-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of White Spot Syndrome Virus Envelope Protein VP51A and Its Interaction with Viral Tegument Protein VP26{triangledown}

Yun-Shiang Chang,1* Wang-Jing Liu,2 Tsung-Lu Chou,1 Yuan-Ting Lee,1 Tai-Lin Lee,1 Wei-Tung Huang,1 Guang-Hsiung Kou,2 and Chu-Fang Lo2*

Department of Molecular Biotechnology, Da-Yeh University, Changhua, Taiwan,1 Institute of Zoology, National Taiwan University, Taipei, Taiwan2

Received 14 June 2008/ Accepted 17 September 2008

In this study, we characterize a novel white spot syndrome virus (WSSV) structural protein, VP51A (WSSV-TW open reading frame 294), identified from a previous mass spectrometry study. Temporal-transcription analysis showed that vp51A is expressed in the late stage of WSSV infection. Gene structure analysis showed that the transcription initiation site of vp51A was 135 bp upstream of the translation start codon. The poly(A) addition signal overlapped with the translation stop codon, TAA, and the poly(A) tail was 23 bp downstream of the TAA. Western blot analysis of viral protein fractions and immunoelectron microscopy both suggested that VP51A is a viral envelope protein. Western blotting of the total proteins extracted from WSSV virions detected a band that was close to the predicted 51-kDa mass, but the strongest signal was around 72 kDa. We concluded that this 72-kDa band was in fact the full-length VP51A protein. Membrane topology assays demonstrated that the VP51A 72-kDa protein is a type II transmembrane protein with a highly hydrophobic transmembrane domain on its N terminus and a C terminus that is exposed on the surface of the virion. Coimmunoprecipitation, colocalization, and yeast two-hybrid assays revealed that VP51A associated directly with VP26 and indirectly with VP28, with VP26 acting as a linker protein in the formation of a VP51A-VP26-VP28 complex.

* Corresponding author. Mailing address for Chu-Fang Lo: Institute of Zoology, National Taiwan University, Taipei 106, Taiwan. Phone: 886-2-33662453. Fax: 886-2-23638179. E-mail: gracelow{at}ntu.edu.tw. Mailing address for Yun-Shiang Chang: Department of Molecular Biotechnology, Da-Yeh University, Changhua 515, Taiwan. Phone: 886-4-8511888, ext. 4265. Fax: 886-4-8511326. E-mail: yschang{at}mail.dyu.edu.tw

{triangledown} Published ahead of print on 1 October 2008.

Journal of Virology, December 2008, p. 12555-12564, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01238-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhou, Q., Xu, L., Li, H., Qi, Y.-P., Yang, F. (2009). Four Major Envelope Proteins of White Spot Syndrome Virus Bind To Form a Complex. J. Virol. 83: 4709-4712 [Abstract] [Full Text]  


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