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Journal of Virology, December 2008, p. 12304-12311, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01696-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of a Movement Protein of the Tenuivirus Rice Stripe Virus{triangledown}

Ruyi Xiong,1,2 Jianxiang Wu,1 Yijun Zhou,2 and Xueping Zhou1*

State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029,1 Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People's Republic of China2

Received 9 August 2008/ Accepted 22 September 2008

Rice stripe virus (RSV) is the type member of the genus Tenuivirus. RSV has four single-stranded RNAs and causes severe disease in rice fields in different parts of China. To date, no reports have described how RSV spreads within host plants or the viral and/or host factor(s) required for tenuivirus movement. We investigated functions of six RSV-encoded proteins using trans-complementation experiments and biolistic bombardment. We demonstrate that NSvc4, encoded by RSV RNA4, supports the intercellular trafficking of a movement-deficient Potato virus X in Nicotiana benthamiana leaves. We also determined that upon biolistic bombardment or agroinfiltration, NSvc4:enhanced green fluorescent protein (eGFP) fusion proteins localize predominantly near or within the walls of onion and tobacco epidermal cells. In addition, the NSvc4:eGFP fusion protein can move from initially bombarded cells to neighboring cells in Nicotiana benthamiana leaves. Immunocytochemistry using tissue sections from RSV-infected rice leaves and an RSV NSvc4-specific antibody showed that the NSvc4 protein accumulated in walls of RSV-infected leaf cells. Gel retardation assays revealed that the NSvc4 protein interacts with single-stranded RNA in vitro, a common feature of many reported plant viral movement proteins (MPs). RSV NSvc4 failed to interact with the RSV nucleocapsid protein using yeast two-hybrid assays. Taken together, our data indicate that RSV NSvc4 is likely an MP of the virus. This is the first report describing a tenuivirus MP.

* Corresponding author. Mailing address: Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China. Phone: 0086 571 86971680. Fax: 0086 571 86971498. E-mail: zzhou{at}zju.edu.cn

{triangledown} Published ahead of print on 25 September 2008.

Journal of Virology, December 2008, p. 12304-12311, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01696-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wei, T.-Y., Yang, J.-G., Liao, F.-L., Gao, F.-L., Lu, L.-M., Zhang, X.-T., Li, F., Wu, Z.-J., Lin, Q.-Y., Xie, L.-H., Lin, H.-X. (2009). Genetic diversity and population structure of rice stripe virus in China. J. Gen. Virol. 90: 1025-1034 [Abstract] [Full Text]  


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