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Journal of Virology, December 2008, p. 11979-11984, Vol. 82, No. 23
0022-538X/08/$08.00+0     doi:10.1128/JVI.00867-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Measles Viruses Possessing the Polymerase Protein Genes of the Edmonston Vaccine Strain Exhibit Attenuated Gene Expression and Growth in Cultured Cells and SLAM Knock-In Mice {triangledown}

Makoto Takeda,1* Shinji Ohno,1 Maino Tahara,1 Hiroki Takeuchi,1 Yuta Shirogane,1 Hirofumi Ohmura,1 Takafumi Nakamura,2,3 and Yusuke Yanagi1

Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan,1 Core Facility for Therapeutic Vectors, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai Minato-ku, Tokyo 108-8639, Japan,2 RNA and Biofunctions, PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan3

Received 24 April 2008/ Accepted 2 September 2008

Live attenuated vaccines against measles have been developed through adaptation of clinical isolates of measles virus (MV) in various cultured cells. Analyses using recombinant MVs with chimeric genomes between wild-type and Edmonston vaccine strains indicated that viruses possessing the polymerase protein genes of the Edmonston strain exhibited attenuated viral gene expression and growth in cultured cells as well as in mice expressing an MV receptor, signaling lymphocyte activation molecule, regardless of whether the virus genome had the wild-type or vaccine-type promoter sequence. These data demonstrate that the polymerase protein genes of the Edmonston strain contribute to its attenuated phenotype.

* Corresponding author. Mailing address: Department of Virology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Phone: 81-92-642-6138. Fax: 81-92-642-6140. E-mail: mtakeda{at}virology.med.kyushu-u.ac.jp

{triangledown} Published ahead of print on 17 September 2008.

Journal of Virology, December 2008, p. 11979-11984, Vol. 82, No. 23
0022-538X/08/$08.00+0     doi:10.1128/JVI.00867-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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