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Journal of Virology, December 2008, p. 11964-11969, Vol. 82, No. 23
0022-538X/08/$08.00+0     doi:10.1128/JVI.01126-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Murine Coronaviruses Encoding nsp2 at Different Genomic Loci Have Altered Replication, Protein Expression, and Localization{triangledown}

Mark J. Gadlage,2,3 Rachel L. Graham,1,3,{dagger} and Mark R. Denison1,2,3*

Departments of Pediatrics,1 Microbiology and Immunology,2 The Elizabeth B. Lamb Center for Pediatric Research, Vanderbilt University Medical Center, Nashville, Tennessee3

Received 23 May 2007/ Accepted 17 September 2008

Partial or complete deletion of several coronavirus nonstructural proteins (nsps), including open reading frame 1a (ORF1a)-encoded nsp2, results in viable mutant proteins with specific replication defects. It is not known whether expression of nsps from alternate locations in the genome can complement replication defects. In this report, we show that the murine hepatitis virus nsp2 sequence was tolerated in ORF1b with an in-frame insertion between nsp13 and nsp14 and in place of ORF4. Alternate encoding or duplication of the nsp2 gene sequence resulted in differences in nsp2 expression, processing, and localization, was neutral or detrimental to replication, and did not complement an ORF1a {Delta}nsp2 replication defect. The results suggest that wild-type genomic organization and expression of nsps are required for optimal replication.

* Corresponding author. Mailing address: Department of Pediatrics, Vanderbilt University Medical Center, D6217 MCN, 1161 21st Ave. S, Nashville, TN 37232. Phone: (615) 343-9881. Fax: (615) 343-9723. E-mail: mark.denison{at}vanderbilt.edu

{triangledown} Published ahead of print on 24 September 2008.

{dagger} Present address: University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7435.

Journal of Virology, December 2008, p. 11964-11969, Vol. 82, No. 23
0022-538X/08/$08.00+0     doi:10.1128/JVI.01126-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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