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Journal of Virology, December 2008, p. 11577-11588, Vol. 82, No. 23
0022-538X/08/$08.00+0     doi:10.1128/JVI.01779-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Simian Immunodeficiency Virus (SIV)-Specific CD8+ T-Cell Responses in Vervet African Green Monkeys Chronically Infected with SIVagm{triangledown}

Roland C. Zahn,1 Melisa D. Rett,1 Birgit Korioth-Schmitz,1 Yue Sun,1 Adam P. Buzby,1 Simoy Goldstein,2 Charles R. Brown,2 Russell A. Byrum,3 Gordon J. Freeman,4 Norman L. Letvin,1 Vanessa M. Hirsch,2 and Jörn E. Schmitz1*

Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115,1 Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892,2 Bioqual, Inc., Rockville, Maryland 20852,3 Department of Medical Oncology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, Boston, Massachusetts 021154

Received 22 August 2008/ Accepted 19 September 2008

African green monkeys (AGM) do not develop overt signs of disease following simian immunodeficiency virus (SIV) infection. While it is still unknown how natural hosts like AGM can cope with this lentivirus infection, a large number of investigations have shown that CD8+ T-cell responses are critical for the containment of AIDS viruses in humans and Asian nonhuman primates. Here we have compared the phenotypes of T-cell subsets and magnitudes of SIV-specific CD8+ T-cell responses in vervet AGM chronically infected with SIVagm and rhesus monkeys (RM) infected with SIVmac. In comparison to RM, vervet AGM exhibited weaker signs of immune activation and associated proliferation of CD8+ T cells as detected by granzyme B, Ki-67, and programmed death 1 staining. By gamma interferon enzyme-linked immunospot assay and intracellular cytokine staining, SIV Gag- and Env-specific immune responses were detectable at variable but lower levels in vervet AGM than in RM. These observations demonstrate that natural hosts like SIV-infected vervet AGM develop SIV-specific T-cell responses, but the disease-free course of infection does not depend on the generation of robust CD8+ T-cell responses.

* Corresponding author. Mailing address: Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Center for Life Sciences, ECLS-1037, 3 Blackfan Circle, Boston, MA 02115. Phone: (617) 735-4475. Fax: (617) 735-4527. E-mail: jschmitz{at}bidmc.harvard.edu

{triangledown} Published ahead of print on 1 October 2008.

Journal of Virology, December 2008, p. 11577-11588, Vol. 82, No. 23
0022-538X/08/$08.00+0     doi:10.1128/JVI.01779-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lozano Reina, J.-M., Favre, D., Kasakow, Z., Mayau, V., Nugeyre, M.-T., Ka, T., Faye, A., Miller, C. J., Scott-Algara, D., McCune, J. M., Barre-Sinoussi, F., Diop, O. M., Muller-Trutwin, M. C. (2009). Gag p27-Specific B- and T-Cell Responses in Simian Immunodeficiency Virus SIVagm-Infected African Green Monkeys. J. Virol. 83: 2770-2777 [Abstract] [Full Text]  


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