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Journal of Virology, November 2008, p. 11472-11475, Vol. 82, No. 22
0022-538X/08/$08.00+0     doi:10.1128/JVI.01086-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Glutamine Deprivation Causes Enhanced Plating Efficiency of a Herpes Simplex Virus Type 1 ICP0-Null Mutant {triangledown}

Ryan M. Bringhurst,1* Antonia A. Dominguez,2 and Priscilla A. Schaffer1,{dagger}

Departments of Medicine and Microbiology and Molecular Genetics, Harvard Medical School at the Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215,1 Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 880032

Received 22 May 2008/ Accepted 27 August 2008

Isoleucine deprivation of cellular monolayers prior to infection has been reported to result in partial complementation of a herpes simplex virus type 1 (HSV-1) ICP0 null (ICP0–) mutant. We now report that glutamine deprivation alone is able to enhance the plating efficiency of an ICP0– virus and that isoleucine deprivation has little or no effect. Because a low glutamine level is associated with stress and because stress is known to induce reactivation, low levels of glutamine may be relevant to the reactivation of HSV-1 from latency. Additionally, we demonstrate that arginine and methionine deprivation result in partial complementation of the ICP0– virus.

* Corresponding author. Present address: University of Arizona, 1007 E. Lowell St., P.O. Box 210106, Tucson, AZ 85721. Phone: (520) 626-4984. Fax: (520) 621-3709. E-mail: rbring{at}email.arizona.edu

{triangledown} Published ahead of print on 3 September 2008.

{dagger} Present address: University of Arizona, 1007 E. Lowell St., P.O. Box 210106, Tucson, AZ 85721.

Journal of Virology, November 2008, p. 11472-11475, Vol. 82, No. 22
0022-538X/08/$08.00+0     doi:10.1128/JVI.01086-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Everett, R. D., Parsy, M.-L., Orr, A. (2009). Analysis of the Functions of Herpes Simplex Virus Type 1 Regulatory Protein ICP0 That Are Critical for Lytic Infection and Derepression of Quiescent Viral Genomes. J. Virol. 83: 4963-4977 [Abstract] [Full Text]  


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