This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vogt, C.
Right arrow Articles by Kochs, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vogt, C.
Right arrow Articles by Kochs, G.

 Previous Article  |  Next Article 

Journal of Virology, November 2008, p. 11446-11453, Vol. 82, No. 22
0022-538X/08/$08.00+0     doi:10.1128/JVI.01284-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The Interferon Antagonist ML Protein of Thogoto Virus Targets General Transcription Factor IIB{triangledown}

Carola Vogt, Ellen Preuss,{dagger} Daniel Mayer, Friedemann Weber, Martin Schwemmle, and Georg Kochs*

Department of Virology, University of Freiburg, Hermann-Herder-Strasse 11, 79104 Freiburg, Germany

Received 20 June 2008/ Accepted 25 August 2008

The ML protein of Thogoto virus, a tick-transmitted orthomyxovirus, is a splice variant of the viral matrix protein and antagonizes the induction of antiviral type I interferon (IFN). Here we identified the general RNA polymerase II transcription factor IIB (TFIIB) as an ML-interacting protein. Overexpression of TFIIB neutralized the inhibitory effect of ML on IRF3-mediated promoter activation. Moreover, a recombinant virus expressing a mutant ML protein unable to bind TFIIB was severely impaired in its ability to suppress IFN induction. We concluded that TFIIB binding is required for the IFN antagonist effect exerted by ML. We further demonstrate that the ML-TFIIB interaction has surprisingly little impact on gene expression in general, while a strong negative effect is observed for IRF3- and NF-{kappa}B-regulated promoters.

* Corresponding author. Mailing address: Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Hermann-Herder-Strasse 11, 79104 Freiburg, Germany. Phone: 49 761 2036623. Fax: 49 761 2036562. E-mail: Georg.kochs{at}uniklinik-freiburg.de

{triangledown} Published ahead of print on 3 September 2008.

{dagger} Present address: Frankfurter Stiftung für krebskranke Kinder, Komturstr. 3a, 60528 Frankfurt am Main, Germany.

Journal of Virology, November 2008, p. 11446-11453, Vol. 82, No. 22
0022-538X/08/$08.00+0     doi:10.1128/JVI.01284-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»