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Journal of Virology, November 2008, p. 10940-10945, Vol. 82, No. 21
0022-538X/08/$08.00+0     doi:10.1128/JVI.01289-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Open Reading Frame 2, Encoded by the Latency-Related Gene of Bovine Herpesvirus 1, Has Antiapoptotic Activity in Transiently Transfected Neuroblastoma Cells{triangledown}

Wenwen Shen and Clinton Jones*

Department of Veterinary and Biomedical Sciences and the Nebraska Center for Virology, University of Nebraska—Lincoln, Fair Street at East Campus Loop, Lincoln, Nebraska, 68583-0905

Received 20 June 2008/ Accepted 8 August 2008

The latency-related (LR) RNA encoded by bovine herpesvirus 1 is abundantly expressed in the trigeminal ganglia of latently infected calves. Expression of LR proteins is necessary for reactivation from latency and the protection of infected neurons from apoptosis. In this study, we demonstrated that an LR-encoded protein, open reading frame 2 (ORF-2), or ORF-2 fusion proteins encoded by alternatively spliced LR transcripts inhibit cold shock or Fas ligand-induced apoptosis in mouse neuroblastoma (neuro-2A) cells. Frameshift mutants of ORF-2 do not inhibit apoptosis, which suggests that protein expression, not LR RNA expression, mediates the antiapoptotic activity of the LR gene in transfected neuro-2A cells.

* Corresponding author. Mailing address: Department of Veterinary and Biomedical Sciences and the Nebraska Center for Virology, University of Nebraska—Lincoln, Fair Street at East Campus Loop, Lincoln, Nebraska 68583-0905. Phone: (402) 472-1890. Fax: (402) 472-9690. E-mail: cjones{at}unlnotes.unl.edu

{triangledown} Published ahead of print on 20 August 2008.

Journal of Virology, November 2008, p. 10940-10945, Vol. 82, No. 21
0022-538X/08/$08.00+0     doi:10.1128/JVI.01289-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shen, W., Sa e Silva, M., Jaber, T., Vitvitskaia, O., Li, S., Henderson, G., Jones, C. (2009). Two Small RNAs Encoded within the First 1.5 Kilobases of the Herpes Simplex Virus Type 1 Latency-Associated Transcript Can Inhibit Productive Infection and Cooperate To Inhibit Apoptosis. J. Virol. 83: 9131-9139 [Abstract] [Full Text]  
  • Workman, A., Perez, S., Doster, A., Jones, C. (2009). Dexamethasone Treatment of Calves Latently Infected with Bovine Herpesvirus 1 Leads to Activation of the bICP0 Early Promoter, in Part by the Cellular Transcription Factor C/EBP-Alpha. J. Virol. 83: 8800-8809 [Abstract] [Full Text]  


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