This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gustems, M.
Right arrow Articles by Angulo, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gustems, M.
Right arrow Articles by Angulo, A.

 Previous Article  |  Next Article 

Journal of Virology, October 2008, p. 10302-10307, Vol. 82, No. 20
0022-538X/08/$08.00+0     doi:10.1128/JVI.01255-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

In Vivo Competence of Murine Cytomegalovirus under the Control of the Human Cytomegalovirus Major Immediate-Early Enhancer in the Establishment of Latency and Reactivation{triangledown} ,{dagger}

Montse Gustems,1 Andreas Busche,2 Martin Messerle,2 Peter Ghazal,3 and Ana Angulo1*

Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona 08036, Spain,1 Department of Virology, Hannover Medical School, Hannover 30625, Germany,2 Division of Pathway Medicine, University of Edinburgh, 49 Little France Crescent, Edinburgh EH16 4SB, United Kingdom3

Received 17 June 2008/ Accepted 29 July 2008

The human cytomegalovirus (HCMV) major immediate-early enhancer has been postulated to play a pivotal role in the control of latency and reactivation. However, the absence of an animal model has obstructed a direct test of this hypothesis. Here we report on the establishment of an in vivo, experimentally tractable system for quantitatively investigating physiological functions of the HCMV enhancer. Using a neonate BALB/c mouse model, we show that a chimeric murine CMV under the control of the HCMV enhancer is competent in vivo, replicating in key organs of mice with acute CMV infection and exhibiting latency/reactivation features comparable for the most part to those of the parental and revertant viruses.

* Corresponding author. Mailing address: Institut d'Investigacions Biomèdiques August Pi i Sunyer, C/Villarroel 170, Barcelona 08036, Spain. Phone: 34 647 450269. Fax: 34 93 4021907. E-mail: aangulo{at}ub.edu

{triangledown} Published ahead of print on 6 August 2008.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, October 2008, p. 10302-10307, Vol. 82, No. 20
0022-538X/08/$08.00+0     doi:10.1128/JVI.01255-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kropp, K. A., Simon, C. O., Fink, A., Renzaho, A., Kuhnapfel, B., Podlech, J., Reddehase, M. J., Grzimek, N. K. A. (2009). Synergism between the components of the bipartite major immediate-early transcriptional enhancer of murine cytomegalovirus does not accelerate virus replication in cell culture and host tissues. J. Gen. Virol. 90: 2395-2401 [Abstract] [Full Text]  
  • Busche, A., Marquardt, A., Bleich, A., Ghazal, P., Angulo, A., Messerle, M. (2009). The Mouse Cytomegalovirus Immediate-Early 1 Gene Is Not Required for Establishment of Latency or for Reactivation in the Lungs. J. Virol. 83: 4030-4038 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»