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Journal of Virology, October 2008, p. 9770-9775, Vol. 82, No. 19
0022-538X/08/$08.00+0     doi:10.1128/JVI.00741-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Basal Budding and Replication of the Murine Leukemia Virus Are Independent of the Gag L Domains{triangledown}

Yosef Sabo, Nihay Laham-Karam, and Eran Bacharach*

Department of Cell Research and Immunology, Tel Aviv University, Tel Aviv 69978, Israel

Received 4 April 2008/ Accepted 20 July 2008

Moloney murine leukemia virus (MMuLV) Gag protein contains three identified late (L) domains, PPPY, YPAL, and PSAP, which are thought to interact with the endosomal sorting machinery to assist budding. We created single and combined L-domain mutants in all permutations and tested the resulting clones for budding and replication. Budding and replication of all viruses with mutated PPPY were greatly reduced; however, the basal replication level was retained, demonstrated by the slow spread of the viruses in culture. Mutations in PSAP or YPAL did not affect budding or spreading, demonstrating that these two motifs are dispensable for efficient MMuLV replication. Furthermore, the basal budding level was maintained following inhibition of endosomal sorting machinery, emphasizing that the basal budding of MMuLV is independent of this machinery.

* Corresponding author. Mailing address: Department of Cell Research and Immunology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel. Phone: 972-3-640-7692. Fax: 972-3-642-2046. E-mail: eranbac{at}post.tau.ac.il

{triangledown} Published ahead of print on 30 July 2008.

Journal of Virology, October 2008, p. 9770-9775, Vol. 82, No. 19
0022-538X/08/$08.00+0     doi:10.1128/JVI.00741-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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