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Journal of Virology, September 2008, p. 9278-9282, Vol. 82, No. 18
0022-538X/08/$08.00+0     doi:10.1128/JVI.00598-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Functional Homologies between Avian and Human Alphaherpesvirus VP22 Proteins in Cell-to-Cell Spreading as Revealed by a New cis-Complementation Assay{triangledown} ,{dagger}

C. Blondeau, D. Marc, K. Courvoisier, J.-F. Vautherot, and C. Denesvre*

Laboratoire de Virologie Moléculaire, INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, Nouzilly F-37380, France

Received 18 March 2008/ Accepted 7 July 2008

VP22, encoded by the UL49 gene of Marek's disease virus (MDV), is indispensable for virus cell-to-cell spreading. We show herein that MDV UL49 can be functionally replaced with avian and human viral orthologs. Replacement of MDV VP22 with that of avian gallid herpesvirus 3 or herpesvirus of turkey, whose residue identity with MDV is close to 60%, resulted in 73 and 131% changes in viral spreading, respectively. In contrast, VP22 replacement with human herpes simplex virus type 1 resulted in 14% plaque formation. Therefore, heterologous avian and human VP22 proteins share sufficient structural homology to support MDV cell-to-cell spreading, albeit with different efficiencies.

* Corresponding author. Mailing address: Laboratoire de Virologie Moléculaire, INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, Nouzilly F-37380, France. Phone: 33-2-47427619. Fax: 33-2-47427774. E-mail: denesvre{at}tours.inra.fr

{triangledown} Published ahead of print on 16 July 2008.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, September 2008, p. 9278-9282, Vol. 82, No. 18
0022-538X/08/$08.00+0     doi:10.1128/JVI.00598-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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