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Journal of Virology, September 2008, p. 9273-9277, Vol. 82, No. 18
0022-538X/08/$08.00+0     doi:10.1128/JVI.00915-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Immune Response in the Absence of Neurovirulence in Mice Infected with M Protein Mutant Vesicular Stomatitis Virus{triangledown}

Maryam Ahmed,1* Tracie R. Marino,1 Shelby Puckett,1 Nancy D. Kock,2 and Douglas S. Lyles1

Departments of Biochemistry,1 Pathology/Comparative Medicine, Wake Forest University School of Medicine, Winston-Salem, North Carolina 271572

Received 2 May 2008/ Accepted 19 June 2008

Matrix (M) protein mutants of vesicular stomatitis virus (VSV), such as rM51R-M virus, are less virulent than wild-type (wt) VSV strains due to their inability to suppress innate immunity. Studies presented here show that when inoculated intranasally into mice, rM51R-M virus was cleared from nasal mucosa by day 2 postinfection and was attenuated for spread to the central nervous system, in contrast to wt VSV, thus accounting for its reduced virulence. However, it stimulated an antibody response similar to that in mice infected with the wt virus, indicating that it has the ability to induce adaptive immunity in vivo without causing disease. These results support the use of M protein mutants of VSV as vaccine vectors.

* Corresponding author. Mailing address: Department of Biochemistry, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157. Phone: (336) 716-1589. Fax: (336) 716-7176. E-mail: mahmed{at}wfubmc.edu

{triangledown} Published ahead of print on 9 July 2008.

Journal of Virology, September 2008, p. 9273-9277, Vol. 82, No. 18
0022-538X/08/$08.00+0     doi:10.1128/JVI.00915-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ozduman, K., Wollmann, G., Ahmadi, S. A., van den Pol, A. N. (2009). Peripheral Immunization Blocks Lethal Actions of Vesicular Stomatitis Virus within the Brain. J. Virol. 83: 11540-11549 [Abstract] [Full Text]  
  • Ahmed, M., Mitchell, L. M., Puckett, S., Brzoza-Lewis, K. L., Lyles, D. S., Hiltbold, E. M. (2009). Vesicular Stomatitis Virus M Protein Mutant Stimulates Maturation of Toll-Like Receptor 7 (TLR7)-Positive Dendritic Cells through TLR-Dependent and -Independent Mechanisms. J. Virol. 83: 2962-2975 [Abstract] [Full Text]  


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