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Journal of Virology, September 2008, p. 8937-8941, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00911-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The Enhanced DNA Replication Fidelity of a Mutant Herpes Simplex Virus Type 1 DNA Polymerase Is Mediated by an Improved Nucleotide Selectivity and Reduced Mismatch Extension Ability{triangledown}

Wang Tian, Ying T. Hwang, and Charles B. C. Hwang*

Department of Microbiology and Immunology, State University of New York, Upstate Medical University, Syracuse, New York 13210

Received 1 May 2008/ Accepted 24 June 2008

We previously demonstrated that a recombinant herpes simplex virus containing a mutation within the finger domain of DNA polymerase replicated DNA with increased fidelity. In this study, we demonstrate that, compared with wild-type polymerase, the mutant enzyme exhibited improved nucleotide selectivity and a reduced ability to extend from mismatched primer termini, which would contribute to the increased DNA replication fidelity.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, SUNY, Upstate Medical University, 750 E. Adams St., Syracuse, NY 13210. Phone: (315) 464-8739. Fax: (315) 464-4417. E-mail: hwangc{at}upstate.edu

{triangledown} Published ahead of print on 2 July 2008.

Journal of Virology, September 2008, p. 8937-8941, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00911-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tian, W., Hwang, Y. T., Lu, Q., Hwang, C. B. C. (2009). Finger Domain Mutation Affects Enzyme Activity, DNA Replication Efficiency, and Fidelity of an Exonuclease-Deficient DNA Polymerase of Herpes Simplex Virus Type 1. J. Virol. 83: 7194-7201 [Abstract] [Full Text]  


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