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Journal of Virology, September 2008, p. 8922-8926, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00493-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Functional F Analogue of Autographa californica Nucleopolyhedrovirus GP64 from the Agrotis segetum Granulovirus{triangledown}

Feifei Yin,1,3 Manli Wang,1,3 Ying Tan,1,3 Fei Deng,1 Just M. Vlak,2 Zhihong Hu,1 and Hualin Wang1*

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China,1 Laboratory of Virology, Wageningen University, 6709 PD Wageningen, The Netherlands,2 Graduate School of the Chinese Academy of Sciences, Beijing 100039, China3

Received 5 March 2008/ Accepted 10 June 2008

The envelope fusion protein F of Plutella xylostella granulovirus is a computational analogue of the GP64 envelope fusion protein of Autographa californica nucleopolyhedrovirus (AcMNPV). Granulovirus (GV) F proteins were thought to be unable to functionally replace GP64 in the AcMNPV pseudotyping system. In the present study the F protein of Agrotis segetum GV (AgseGV) was identified experimentally as the first functional GP64 analogue from GVs. AgseF can rescue virion propagation and infectivity of gp64-null AcMNPV. The AgseF-pseudotyped AcMNPV also induced syncytium formation as a consequence of low-pH-induced membrane fusion.

* Corresponding author. Mailing address: Wuhan Institute of Virology, CAS, Wuhan 430071, People's Republic of China. Phone and fax: 86-27-87199353. E-mail: h.wang{at}wh.iov.cn

{triangledown} Published ahead of print on 18 June 2008.

Journal of Virology, September 2008, p. 8922-8926, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00493-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yu, I.-L., Bray, D., Lin, Y.-C., Lung, O. (2009). Autographa californica multiple nucleopolyhedrovirus ORF 23 null mutant produces occlusion-derived virions with fewer nucleocapsids. J. Gen. Virol. 90: 1499-1504 [Abstract] [Full Text]  


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