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Journal of Virology, September 2008, p. 8917-8921, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.02362-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Translation Termination Reinitiation between Open Reading Frame 1 (ORF1) and ORF2 Enables Capsid Expression in a Bovine Norovirus without the Need for Production of Viral Subgenomic RNA{triangledown} ,{dagger}

Christopher J. McCormick,* Omar Salim, Paul R. Lambden, and Ian N. Clarke

Molecular Microbiology Group, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom

Received 1 November 2007/ Accepted 13 June 2008

A generally accepted view of norovirus replication is that capsid expression requires production of a subgenomic transcript, the presence of capsid often being used as a surrogate marker to indicate the occurrence of viral replication. Using a polymerase II-based baculovirus delivery system, we observed capsid expression following introduction of a full-length genogroup 3 norovirus genome into HepG2 cells. However, capsid expression occurred as a result of a novel translation termination/reinitiation event between the nonstructural-protein and capsid open reading frames, a feature that may be unique to genogroup 3 noroviruses.

* Corresponding author. Mailing address: MP814, South Academic Block, Southampton General Hospital, Southampton SO16 6YD, United Kingdom. Phone: 44-2380777222, ext. 3498. Fax: 44-2380796992. E-mail: cjm{at}soton.ac.uk

{triangledown} Published ahead of print on 25 June 2008.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, September 2008, p. 8917-8921, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.02362-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Guo, L.-h., Sun, L., Chiba, S., Araki, H., Suzuki, N. (2009). Coupled termination/reinitiation for translation of the downstream open reading frame B of the prototypic hypovirus CHV1-EP713. Nucleic Acids Res 37: 3645-3659 [Abstract] [Full Text]  
  • Luttermann, C., Meyers, G. (2009). The importance of inter- and intramolecular base pairing for translation reinitiation on a eukaryotic bicistronic mRNA. Genes Dev. 23: 331-344 [Abstract] [Full Text]  


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