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Journal of Virology, September 2008, p. 8911-8916, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00672-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of the Heparin-Dependent Transduction Domain on the Capsid of a Novel Adeno-Associated Virus Isolate, AAV(VR-942){triangledown}

Michael Schmidt,1 Lakshmanan Govindasamy,2 Sandra Afione,1 Nick Kaludov,1 Mavis Agbandje-McKenna,2* and John A. Chiorini1*

Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892,1 Department of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, Florida 3261012

Received 26 March 2008/ Accepted 27 May 2008

A new adeno-associated virus (AAV), referred to as AAV(VR-942), has been isolated as a contaminant of adenovirus strain simian virus 17. The sequence of the rep gene places it in the AAV serotype 2 (AAV2) complementation group, while the capsid is only 88% identical to that of AAV2. High-level AAV(VR-942) transduction activity requires cell surface heparan sulfate proteoglycans, although AAV(VR-942) lacks residues equivalent to the AAV2 R585 and R588 amino acid residues essential for mediating the interaction of AAV2 with the heparan sulfate proteoglycan receptor. Instead, AAV(VR-942) uses a distinct transduction region. This finding shows that distinct domains on different AAV isolates can be responsible for the same activities.

* Corresponding author. Mailing address for John A. Chiorini: NIH 10/1N113, 10 Center Dr., MSC1190, Bethesda, MD 20892. Phone: (301) 496-4279. Fax: (301) 402-1228. E-mail: jchiorini{at}dir.nidcr.nih.gov. Mailing address for Mavis Agbandje-McKenna: Department of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, FL 32610. Phone: (352) 392-5694. Fax: (352) 392-3422. E-mail: mckenna{at}ufl.edu

{triangledown} Published ahead of print on 4 June 2008.

Journal of Virology, September 2008, p. 8911-8916, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00672-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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