This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haupt, S.
Right arrow Articles by Schmidt, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haupt, S.
Right arrow Articles by Schmidt, B.

 Previous Article  |  Next Article 

Journal of Virology, September 2008, p. 8900-8905, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00196-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

CD4 Binding Affinity Determines Human Immunodeficiency Virus Type 1-Induced Alpha Interferon Production in Plasmacytoid Dendritic Cells {triangledown}

Sabrina Haupt,1 Norbert Donhauser,1 Chawaree Chaipan,1 Philipp Schuster,1 Bridget Puffer,2,{dagger} Rod S. Daniels,3 Thomas C. Greenough,4 Frank Kirchhoff,5 and Barbara Schmidt1*

Institute of Clinical and Molecular Virology, German National Reference Centre for Retroviruses, University of Erlangen-Nürnberg, Erlangen, Germany,1 Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania,2 Virology Division, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom,3 Department of Pediatrics, University of Massachusetts Medical School, Worcester, Massachusetts,4 Institute of Virology, University of Ulm, Ulm, Germany5

Received 28 January 2008/ Accepted 11 June 2008

Plasmacytoid dendritic cells (PDC) are major producers of type I interferons (IFN) in response to human immunodeficiency virus type 1 (HIV-1) infection. To better define the underlying mechanisms, we studied the magnitude of alpha IFN (IFN-{alpha}) induction by recombinant viruses containing changes in the Env protein that impair or disrupt CD4 binding or expressing primary env alleles with differential coreceptor tropism. We found that the CD4 binding affinity but not the viral coreceptor usage is critical for the attachment of autofluorescing HIV-1 to PDC and for subsequent IFN-{alpha} induction. Our results illustrate the importance of the gp120-CD4 interaction in determining HIV-1-induced immune stimulation via IFN-{alpha} production.

* Corresponding author. Mailing address: Institute of Clinical and Molecular Virology, German National Reference Centre for Retroviruses, University of Erlangen-Nürnberg, Schlossgarten 4, D-91054 Erlangen, Germany. Phone: 49-9131-85-25790. Fax: 49-9131-85-26485. E-mail: baschmid{at}viro.med.uni-erlangen.de

{triangledown} Published ahead of print on 25 June 2008.

{dagger} Present address: Integral Molecular, Philadelphia, PA.

Journal of Virology, September 2008, p. 8900-8905, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00196-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»