This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schaumburg, C.
Right arrow Articles by Atwood, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schaumburg, C.
Right arrow Articles by Atwood, W. J.

 Previous Article  |  Next Article 

Journal of Virology, September 2008, p. 8896-8899, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00406-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cells Express the Serotonin Receptor and Are Susceptible to JC Virus Infection{triangledown}

C. Schaumburg,1,2,{ddagger} B. A. O'Hara,3,{ddagger} T. E. Lane,1,2 and W. J. Atwood3*

Department of Molecular Biology and Biochemistry, University of California, Irvine, California,1 Sue and Bill Gross Stem Cell Research Center, Irvine, California 92697,2 Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 029123

Received 25 February 2008/ Accepted 17 June 2008

We studied the susceptibility of human embryonic stem cell-derived oligodendrocyte progenitor cells to infection with JC virus, the causative agent of progressive multifocal leukoencephalopathy (PML). A human embryonic stem cell line, H7, was used to derive an enriched population of cells expressing the oligodendrocyte progenitor cell-specific marker NG2. These cells expressed the 5HT2a receptor (5HT2aR) for JC virus and were highly susceptible to infection. Infection was reduced by treatment with anti-5HT2aR antibodies and by the 5HT2aR antagonists ritanserin and ketanserin. This is the first demonstration that human embryonic stem cell-derived oligodendrocyte progenitor cells are susceptible to JC virus infection and indicates that cells poised to replenish mature oligodendrocytes in PML lesions may also be a target of viral infection.

* Corresponding author. Mailing address: Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 70 Ship Street, Box G-E434, Providence, RI 02903. Phone: (401) 863-3116. Fax: (401) 863-9653. E-mail: Walter_Atwood{at}Brown.edu

{triangledown} Published ahead of print on 25 June 2008.

{ddagger} These authors contributed equally to this work.

Journal of Virology, September 2008, p. 8896-8899, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00406-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»