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Journal of Virology, September 2008, p. 8392-8399, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00951-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Nuclear Pore Composition and Gating in Herpes Simplex Virus-Infected Cells{triangledown}

Helmut Hofemeister{dagger} and Peter O'Hare*

Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom

Received 7 May 2008/ Accepted 11 June 2008

The mechanism by which herpes simplex virus (HSV) exits the nucleus remains a matter of controversy. The generally accepted route proposes that capsids exit via primary envelopment at the inner nuclear membrane and subsequent fusion of this primary particle with the outer nuclear membrane to gain capsid entry to the cytoplasm. However, recent observations indicate that HSV may induce gross morphological alterations of nuclear pores, resulting in the loss of normal pores and the appearance of dilated gaps in the nuclear membrane of up to several 100 nm. On this basis, it was proposed that a main route of capsid exit from the nucleus is directly through these altered pores. Here, we examine the biochemical composition of some of the major nuclear pore components in uninfected and HSV-infected cells. We show that total levels of major nucleoporins and their sedimentation patterns in density gradients remain largely unchanged up to 18 h after HSV infection. Some alteration in modification of one nucleoporin, Nup358/RanBP2, was observed during enrichment with anti-nucleoporin antibody and probing for O glycosylation. In addition, we examine functional gating within the nucleus in live cells, using microinjection of labeled dextran beads and a recombinant virus expressing GFP-VP16 to track the progress of infection. The nuclear permeability barrier for molecules bigger than 70 kDa remained intact throughout infection. Thus, in a functional assay in live cells, we find no evidence for gross perturbation to the gating of nuclear pores, although this might not exclude a small population of modified pores.

* Corresponding author. Mailing address: Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom. Phone: 44 01883 722 306. Fax: 44 01883 714 375. E-mail: P.OHare{at}mcri.ac.uk

{triangledown} Published ahead of print on 18 June 2008.

{dagger} Present address: BIOTEC, TU-Dresden, Germany.

Journal of Virology, September 2008, p. 8392-8399, Vol. 82, No. 17
0022-538X/08/$08.00+0     doi:10.1128/JVI.00951-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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