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Journal of Virology, August 2008, p. 8215-8223, Vol. 82, No. 16
0022-538X/08/$08.00+0     doi:10.1128/JVI.02575-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Liver-Specific MicroRNA miR-122 Enhances the Replication of Hepatitis C Virus in Nonhepatic Cells{triangledown}

Jinhong Chang,1,§* Ju-Tao Guo,1,§ Dong Jiang,1 Haitao Guo,1 John M. Taylor,3 and Timothy M. Block1,2

Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine,1 Institute for Hepatitis and Virus Research, Hepatitis B Foundation, 3805 Old Easton Road, Doylestown, Pennsylvania 18902,2 Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, Pennsylvania 191113

Received 3 December 2007/ Accepted 4 June 2008

The liver-specific microRNA miR-122 has been shown to be required for the replication of hepatitis C virus (HCV) in the hepatoma cell line Huh7. The aim of this study was to test if HCV replication can be modulated by exogenously expressed miR-122 in human embryonic kidney epithelial cells (HEK-293). Our results demonstrate that miR-122 enhances the colony formation efficiency of the HCV replicon and increases the steady-state level of HCV RNA in HEK-293 cells. Therefore, we conclude that although miR-122 is not absolutely required, it greatly enhances HCV replication in nonhepatic cells.

* Corresponding author. Mailing address: Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902. Phone: (215) 589-6325. Fax: (215) 489-4920. E-mail: jinhong.chang{at}drexelmed.edu

{triangledown} Published ahead of print on 11 June 2008.

§ J. Chang and J.-T. Guo contributed equally to this work.

Journal of Virology, August 2008, p. 8215-8223, Vol. 82, No. 16
0022-538X/08/$08.00+0     doi:10.1128/JVI.02575-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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