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Journal of Virology, August 2008, p. 7752-7756, Vol. 82, No. 15
0022-538X/08/$08.00+0     doi:10.1128/JVI.01003-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Vpx Is Critical for Reverse Transcription of the Human Immunodeficiency Virus Type 2 Genome in Macrophages{triangledown}

Mikako Fujita,1 Masami Otsuka,1 Masami Miyoshi,2 Boonruang Khamsri,2 Masako Nomaguchi,2 and Akio Adachi2*

Department of Bioorganic Medicinal Chemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862-0973, Japan,1 Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan2

Received 8 May 2007/ Accepted 8 May 2008

The abilities of wild-type and vpx-defective human immunodeficiency virus type 2 (HIV-2) clones to synthesize viral DNA in human monocyte-derived macrophages (MDMs) and lymphocytic cells were comparatively and quantitatively evaluated. While the vpx-defective mutant directed the synthesis of viral DNA comparably to the wild-type virus and normally in lymphocytic cells, no appreciable viral DNA was detected in MDMs infected with the mutant. To substantiate this finding and to determine whether there is some specific region(s) in Vpx crucial for viral DNA synthesis in MDMs, we generated a series of site-specific point mutants of vpx and examined their phenotypes. The resultant five mutants, with no infectivity for MDMs, showed, without exception, the same defect as the vpx-defective mutant. Our results here clearly demonstrated that the entire Vpx protein is critical for reverse transcription of the HIV-2 genome in human MDMs.

* Corresponding author. Mailing address: Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima-shi, Tokushima 770-8503, Japan. Phone: 81-88-633-7078. Fax: 81-88-633-7080. E-mail: adachi{at}basic.med.tokushima-u.ac.jp

{triangledown} Published ahead of print on 21 May 2008.

Journal of Virology, August 2008, p. 7752-7756, Vol. 82, No. 15
0022-538X/08/$08.00+0     doi:10.1128/JVI.01003-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Goujon, C., Arfi, V., Pertel, T., Luban, J., Lienard, J., Rigal, D., Darlix, J.-L., Cimarelli, A. (2008). Characterization of Simian Immunodeficiency Virus SIVSM/Human Immunodeficiency Virus Type 2 Vpx Function in Human Myeloid Cells. J. Virol. 82: 12335-12345 [Abstract] [Full Text]  


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