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Journal of Virology, August 2008, p. 7388-7394, Vol. 82, No. 15
0022-538X/08/$08.00+0     doi:10.1128/JVI.00225-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

UL36p Is Required for Efficient Transport of Membrane-Associated Herpes Simplex Virus Type 1 along Microtubules{triangledown}

Sara K. Shanda and Duncan W. Wilson*

Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, New York 10461

Received 1 February 2008/ Accepted 13 May 2008

Microtubule-mediated anterograde transport is essential for the transport of herpes simplex virus type 1 (HSV-1) along axons, yet little is known regarding the mechanism and the machinery required for this process. Previously, we were able to reconstitute anterograde transport of HSV-1 on microtubules in an in vitro microchamber assay. Here we report that the large tegument protein UL36p is essential for this trafficking. Using a fluorescently labeled UL36 null HSV-1 strain, K{Delta}UL36GFP, we found that it is possible to isolate a membrane-associated population of this virus. Although these viral particles contained normal amounts of tegument proteins VP16, vhs, and VP22, they displayed a 3-log decrease in infectivity and showed a different morphology compared to UL36p-containing virions. Membrane-associated K{Delta}UL36GFP also displayed a slightly decreased binding to microtubules in our microchamber assay and a two-thirds decrease in the frequency of motility. This decrease in binding and motility was restored when UL36p was supplied in trans by a complementing cell line. These findings suggest that UL36p is necessary for HSV-1 anterograde transport.

* Corresponding author. Mailing address: Department of Developmental and Molecular Biology, 1300 Morris Park Ave., Bronx, NY 10461. Phone: (718) 430-2305. Fax: (718) 430-8567. E-mail: wilson{at}aecom.yu.edu

{triangledown} Published ahead of print on 21 May 2008.

Journal of Virology, August 2008, p. 7388-7394, Vol. 82, No. 15
0022-538X/08/$08.00+0     doi:10.1128/JVI.00225-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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